Results 101 to 110 of about 22,410 (289)

Additive‐Manufactured, Multifunctional Bioreactor Technology for Dynamic Culture of 3D Bioprinted Tissue Models

Advanced Materials Technologies, EarlyView.
We introduce an additively manufactured bioreactor with a perfusion flow system and integrated temperature and pH sensors for skeletal muscle tissue biofabrication. The bioreactor's performance was evaluated by assessing the viability, spreading of the myoblast cells in a printed scaffold, and contraction of the isolated murine musculi interossei ...
Lys Sprenger, Johann Schorzmann, Hsuan‐Heng Lu, Muhammad Osama, Julian Bauer, Michael Haug, Oliver Friedrich, Aldo R. Boccaccini, Frank Döpper, Sahar Salehi   +9 more
wiley   +1 more source

Tailoring Vascular‐Immune Homeostasis via Manganese‐DNA Complex‐Armed Immunogenic Extracellular Vesicles for Pancreatic Cancer Immunotherapy

Advanced Science, EarlyView.
This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang, Lihuan Shang, Xiaochun Chen, Xiangzhan Kong, Xiaojuan Wang, Linjia Jiang, Huiying Zhao, Yafeng Zhu, Cheng Huang, Shiyi Deng, Rui Zhang, Minghui Wang, Haoming Lin, Ping‐Pui Wong   +13 more
wiley   +1 more source

High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

Microbial Cell Factories, 2011
This report describes the combined use of an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant
Ukkonen Kaisa, Vasala Antti, Ojamo Heikki, Neubauer Peter   +3 more
doaj   +1 more source

Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl‐tRNA Synthetase Boosts Genetic Code Expansion in E. coli

Advanced Science, EarlyView.
ABSTRACT Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification.
Nikolaj G. Koch, Peter Goettig, Michael A. Nash, Juri Rappsilber, Nediljko Budisa   +4 more
wiley   +1 more source

Mechanostimulatory Cues Determine Intestinal Fibroblast Fate and Profibrotic Remodeling in a Physiodynamic Human Gut‐on‐a‐Chip

Advanced Science, EarlyView.
A healthy gut barrier shields underlying fibroblasts from luminal shear forces, illustrating that “good fences make good neighbors.” Barrier damage exposes fibroblasts to shear stress, inducing cell death and the emergence of stress‐adapted, profibrotic fibroblasts. Sustained shear exposure promotes the formation of stiff aggregates of mechanoadapative
Soyoun Min, Nam Than, Yong Cheol Shin, Elif G. Ertugral, Chandrasekhar R. Kothapalli, Olumuyiwa Awoniyi, Hyun Jung Kim   +6 more
wiley   +1 more source

Quantitative proteomic profiling of shake flask versus bioreactor growth reveals distinct responses of Agrobacterium tumefaciens for preparation in molecular pharming

, 2020
The preparation of Agrobacterium tumefaciens cultures with strains encoding proteins intended for therapeutic or industrial purposes is an important activity prior to treatment of plants for transient expression of valuable protein products.
McLean, Michael D., Geddes-McAlister, Jennifer, Cossar, Doug, Pastora, Rebecca, Prudhomme, Nicholas, Gianetto-Hill, Connor, Cheung, Wing-Fai, Allen-Vercoe, Emma   +7 more
core   +1 more source

Critical comparison of shake-flask, potentiometric and chromatographic methods for lipophilicity evaluation (log Po/w) of neutral, acidic, basic, amphoteric, and zwitterionic drugs [PDF]

, 2019
In the present study three different procedures have been compared for the determination of the lipophilicity of the unionized species (log Po/w) of neutral, acidic, basic, amphoteric, and zwitterionic drugs.
Subirats i Vila, Xavier, Rosés Pascual, Martí, Bordas, Magda, Ràfols Llach, Clara, Port, Ariadna, Bosch, Elisabeth, Enrech, Raquel, Pascual, Rosalía   +7 more
core   +1 more source

Influence of nitrogen sources on growth of thraustochytrids in waste water from the demineralization of cheese whey

Czech Journal of Food Sciences, 2019
An experimental design was ued to optimize the growth of two thraustochytrids, (Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4), on different nitrogen sources (yeast extract, corn steep liquor, ammonium sulphate) supplemented into saline
Tomáš Humhal, Olga Kronusová, Petr Kaštánek, Tomáš Potočár, Jana Kohoutková, Tomáš Brányik   +5 more
doaj   +1 more source

A Microbial Lipid‐ATP Synthase Axis Fuels NK Cell Antitumor Activity

Advanced Science, EarlyView.
This study focuses on the mechanism by which gut microbiota‐derived outer membrane vesicles (OMVs) regulate NK cell antitumor activity. B. intestinalis is identified to decrease extra‐intestinal tumor growth via its OMVs enriched in sphingosine (SP).
Kaiyuan Yu, Xinyu Sun, Wanxia Ma, Jianming Yang, Xuan Sun, Lisong Zhang, Yumeng Liu, Tianshu Ren, Qi Wang, Jingyu Wang, Xiao Li, Xianping Peng, Liu Yang, Junqiang Lv, Zhi Yao, Zhi‐Song Zhang, Quan Wang   +16 more
wiley   +1 more source

Fermentation optimization of maltose-binding protein fused to neutrophil-activating protein from Escherichia coli TB1

Electronic Journal of Biotechnology, 2015
Background: The fermentation conditions of recombinant maltose-binding protein fused to neutrophil-activating protein (rMBP-NAP) of Helicobacter pylori were optimized from Escherichia coli TB1 with varying medium, inoculum age and size, time, inducer, pH
Jike Lu, Qi Song, Zhenyu Ji, Xin Liu, Ting Wang, Qiaozhen Kang   +5 more
doaj   +1 more source