Results 161 to 170 of about 17,254 (263)
Chemistry – A European Journal, Volume 28, Issue 67, December 1, 2022., 2022 Structural and biochemical data demonstrate that 4‐phenylbenzoic acid binds within the active site of the cytochrome P450 monooxygenase CYP199A4, but no oxidation occurs. Protein engineering and molecular dynamic simulations establish that aromatic oxidation is enabled by a subtle shift of the substrate relative to the heme, thus improving our ...
Tom Coleman +9 more
wiley +1 more source
Phenotyping the quality of complex medium components by simple online-monitored shake flask experiments. [PDF]
Microb Cell Fact, 2014 Diederichs S +6 more
europepmc +1 more source
Specific Lipid Studies in Complex Membranes by Solid‐State NMR Spectroscopy
Chemistry – A European Journal, Volume 28, Issue 70, December 15, 2022., 2022 Specific lipid interaction in complex membranes: Specific interactions with lipids are critical for proteins and drugs, however, direct structural measurements of interactions with lipids remain difficult, especially in membranes that mimic biological conditions.
Roy A. M. van Beekveld +7 more
wiley +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Protein developability is an important, yet often overlooked, aspect of protein discovery campaigns that is a key driver of utility. Recent advances have improved developability screening capacity, making it an increasingly viable option in early‐stage discovery.
Gregory H. Nielsen +2 more
wiley +1 more source
The Effect of Certain Mineral Elements on the Production of Penicillin in Shake Flasks [PDF]
Journal of Bacteriology, 1947 W. C. Frazier, H. Koffler, S. G. Knight
openaire +3 more sources
Sigma Factors as Potential Targets to Enhance Recombinant Protein Expression
Biotechnology and Bioengineering, EarlyView.ABSTRACT The transcriptional factors control the expression of many genes and represent an important layer of complexity in cell factories. However, the effect of individual sigma factor deletions from a biomanufacturing perspective has not been addressed. In this contribution, growth, green fluorescence protein (GFP) expression, and oxygen consumption
Laura Pohlen +6 more
wiley +1 more source
Study on standardization of shake-flask solubility determination method
European Journal of Pharmaceutical Sciences, 2007 E. Baka, Krisztina Takács-Novák
openaire +2 more sources
Perfusion‐Based Production of rAAV via an Intensified Transient Transfection Process
Biotechnology and Bioengineering, EarlyView.ABSTRACT Increasing demand for recombinant adeno‐associated virus (rAAV)‐based gene therapies necessitates increased manufacturing production. Transient transfection of mammalian cells remains the most commonly used method to produce clinical‐grade rAAVs due to its ease of implementation.
Tam N. T. Nguyen +11 more
wiley +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Escherichia coli accumulates acetate as a byproduct in fast growth aerobic conditions when using glucose as carbon source. This phenomenon, known as overflow metabolism, has negative impacts on cell growth and protein expression, also causes carbon loss during biosynthesis in most microbial production scenarios. In this study, we regarded the “
Jianli Zhang +8 more
wiley +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Recombinant adeno associated virus (rAAV) vectors have become popular delivery vehicles for in vivo gene therapies, but demand for rAAVs continues to outpace supply. Platform processes for rAAV production are being developed by many manufacturers, and transient chemical transfection of human embryonic kidney 293 (HEK293) cells is currently the
Erica A. Green +9 more
wiley +1 more source