Results 171 to 180 of about 17,254 (263)

Physiology and Robustness of Yeasts Exposed to Dynamic pH and Glucose Environments

Biotechnology and Bioengineering, EarlyView.
The authors investigated how three Saccharomyces cerevisiae strains respond to dynamic substrate and pH oscillations using dynamic microfluidic single‐cell cultivation (dMSCC) and intracellular biosensors. By combining performance and robustness analysis of selected intracellular parameters, growth and morphological analysis, the authors identified ...
Luca Torello Pianale, Luisa Blöbaum, Alexander Grünberger, Lisbeth Olsson   +3 more
wiley   +1 more source

Toward Sustainable Polyhydroxyalkanoates: A Next-Gen Biotechnology Approach. [PDF]

Polymers (Basel)
Kalia VC, Singh RV, Gong C, Lee JK.
europepmc   +1 more source

Systematic Investigation of Impact of Antifoam and Extracellular Vesicles on Fouling of Hollow Fiber Filters in Intensified Perfusion Processes Highlights the Key Impact of Antifoam

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Ultra‐high cell density perfusion cultures are becoming increasingly attractive for biologics manufacturing due to their ability to boost productivity and offer a flexible manufacturing footprint. However, these intensified perfusion processes pose challenges, particularly concerning the performance of hollow fiber filters used as cell ...
Sri Ranganayaki Madabhushi, Qianying Zuo, Carol Zhou, Chelsea Phuangthong, Nathaniel Ruppert, Tiffany Tang, Francisco Pelaez   +6 more
wiley   +1 more source

Altering 15‐Lipoxygenases to 18‐Lipoxygenases and Their Application to the Production of 5,18‐Dihydroxyeicosapentaenoic Acids

Biotechnology and Bioengineering, EarlyView.
15R‐ and 15S‐lipoxygenases (15R‐ and 15S‐LOXs) were engineered into 18R‐ and 18S‐LOXs, respectively. Escherichia coli expressing these enzymes converted eicosapentaenoic acid into 18R‐ and 18S‐hydroxyeicosapentaenoic acids (18R‐ and 18S‐HEPEs), respectively.
Jin Lee, Su‐Hwan Kang, Tae‐Eui Lee, Deok‐Kun Oh   +3 more
wiley   +1 more source

A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy. [PDF]

J Biol Eng
Schulte A, Brockmann J, Müller N, Anderlei T, Büchs J.   +4 more
europepmc   +1 more source

Efficient One‐Step Production of 7S,17S‐ and 10S,17S‐Dihydroxydocosahexaenoic Acids by a Double‐Oxygenating 15S‐Lipoxygenase From Chlamydomonas incerta

Biotechnology and Bioengineering, EarlyView.
Biotransformation pathway from polyunsaturated fatty acids into dihydroxy fatty acids as lipid mediators by Escherichia coli expressing Chlamydomonas incerta 15S‐lipoxygenase with double oxygenating activity. The 15S‐lipoxygenase was the most efficient for the simultaneous production of resolvin D5 (7S,17S‐dihydroxydocosahexaenoic acid, 7S,17S‐DiHDHA ...
Hyun‐Ah Park, Jin Lee, Deok‐Kun Oh
wiley   +1 more source

Temporal Galactose‐Manganese Feeding in Fed‐Batch and Perfusion Bioreactors Modulates UDP‐Galactose Pools for Enhanced mAb Glycosylation Homogeneity

Biotechnology and Bioengineering, EarlyView.
This study investigates the temporal effects of galactose and manganese on the N‐glycosylation of Trastuzumab (mAb) antibody during fed‐batch and perfusion bioprocessing modes to reveal the influence of feeding schedules on mAb N‐glycosylation patterns. These findings will aid in building process control schemes for mAb bioprocessing.
Aron Gyorgypal, Erica Fratz‐Berilla, Casey Kohnhorst, David N. Powers, Shishir P. S. Chundawat   +4 more
wiley   +1 more source

System metabolic engineering modification of Saccharomyces cerevisiae to increase SAM production. [PDF]

Bioresour Bioprocess
Tan L, Zhang Y, Liu P, Wu Y, Huang Z, Hu Z, Liu Z, Wang Y, Zheng Y.   +8 more
europepmc   +1 more source

Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations

Biotechnology Progress, EarlyView.
Abstract The possibility to produce recombinant adeno‐associated virus (rAAV) by adherent HEK293T cells was studied in a stirred tank bioreactor (STR) culture of cell aggregates. A proof‐of‐concept of rAAV production was successfully demonstrated in a process where single cells were first expanded, then cell aggregates were formed by dilution into a ...
Brian Ladd, Torbjörn Gräslund, Véronique Chotteau   +2 more
wiley   +1 more source

Advances in synthesizing plant-derived isoflavones and their precursors with multiple pharmacological activities using engineered yeasts. [PDF]

Microb Cell Fact
Niu W, Zhang J, Qu L, Ji XJ, Wei Y.
europepmc   +1 more source