Results 181 to 190 of about 17,254 (263)

Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell concentrations

Biotechnology Progress, EarlyView.
Abstract The emergence of new viruses and the spread of existing pathogens necessitate efficient vaccine production methods. The baculovirus expression vector system (BEVS) is an efficient and scalable system for subunit and virus‐like particle vaccine production and gene therapy vectors.
Jort J. Altenburg, Brenda E. Juarez‐Garza, Jelle van Keimpema, Linda van Oosten, Gorben P. Pijlman, Monique M. van Oers, René H. Wijffels, Dirk E. Martens   +7 more
wiley   +1 more source

Insights into the morphology‐productivity relationship of filamentous fungi through small‐scale cultivation and automated microscopy of Thermothelomyces thermophilus

Biotechnology Progress, EarlyView.
Abstract Filamentous fungi are a cornerstone in the biotechnological production of enzymes, proteins, and organic acids. However, challenges in understanding and controlling the relationship between morphology and productivity can limit their application.
Katja Rohr, Bertram Geinitz, Johannes Seiffarth, Aydin Anbarani, Sören Bernauer, Matthias Moch, Julia Tenhaef, Wolfgang Wiechert, Katharina Nöh, Marco Oldiges   +9 more
wiley   +1 more source

Scale-up of CHO cell cultures: from 96-well-microtiter plates to stirred tank reactors across three orders of magnitude. [PDF]

J Biol Eng
Neuss A, Steimann T, Tomas Borges JS, Dinger R, Magnus JB.   +4 more
europepmc   +1 more source

A method for facile production of variable lymphocyte receptors using SHuffle Escherichia coli

Biotechnology Progress, EarlyView.
Abstract Variable lymphocyte receptors (VLRs) are the antigen receptors of jawless vertebrates such as lamprey. VLRs are of growing biotechnological interest for their ability to bind certain antigenic targets with higher affinity than traditional immunoglobulins.
Elizabeth A. Appelt, James B. Thoden, Candis Dancy, Hannah D. Bachmeier, Seth A. Gehrke, Melanie A. Staffenson, Ivan Rayment, Moriah E. Katt, Hazel M. Holden, Eric V. Shusta   +9 more
wiley   +1 more source

Bioprocess exploitation of microaerobic auto-induction using the example of rhamnolipid biosynthesis in Pseudomonas putida KT2440. [PDF]

J Biol Eng
Grether J, Dittmann H, Willems L, Schmiegelt T, Benatto Perino EH, Hubel P, Lilge L, Hausmann R.   +7 more
europepmc   +1 more source

Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding

Biotechnology Progress, EarlyView.
Abstract The production of disulfide‐containing recombinant proteins often requires refolding of inclusion bodies before purification. A pre‐refolding purification step is crucial for effective refolding because impurities in the inclusion bodies interfere with refolding and subsequent purification.
Shuichiro Kimura, Wataru Yamamoto, Ai Miyamoto, Koreyoshi Imamura, Junichiro Futami   +4 more
wiley   +1 more source

Overproduction of endusamycin in <i>Streptomyces endus</i> subsp. <i>aureus</i>. [PDF]

Synth Syst Biotechnol
Chang Y, Liu Z, Deng Z, Liu T.
europepmc   +1 more source

Improving outcomes in intensified processing via optimization of the cell line development workflow

Biotechnology Progress, EarlyView.
Abstract As the industry continues to explore the benefits of continuous and intensified manufacturing, it is important to assure that the cell line development (CLD) workflows in practice today are well suited to generate clones that meet the unique challenges associated with these processes. Most cell lines used in intensified processes are currently
Vincent Balassi, Mary Otto, Corey Kretzmer, Amber Petersen, Channing McLaurin, Jana Mahadevan, Jason Gustin, Trissa Borgschulte, David Razafsky   +8 more
wiley   +1 more source

Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments

Biotechnology Progress, EarlyView.
Abstract The quality control of RNA has become increasingly crucial with the rise of mRNA‐based vaccines and therapeutics. However, conventional methods such as LC–MS often require specialized equipment and expertise, limiting their applicability to high throughput experiments.
Rochelle Aw, Karen Polizzi
wiley   +1 more source

Metal Ion Supplementation to Boost Melanin Production by Streptomyces nashvillensis. [PDF]

Int J Mol Sci
Restaino OF, Kordjazi T, Tancredi F, Manini P, Lanzillo F, Raganati F, Marzocchella A, Porta R, Mariniello L.   +8 more
europepmc   +1 more source