Biotechnology Progress, EarlyView.For biofuels production, hemicellulose pre‐hydrolysate is considered as an attractive feedstock rich in fermentable sugars. The pre‐hydrolysate derived from lignocellulosic biomass comprises, along with sugars, several inhibitors which can hamper its efficient conversion.
Mariem Theiri +3 more
wiley +1 more source
Evaluation of gene editing in CHO cells using the Cas‐CLOVER system
Biotechnology Progress, EarlyView.Abstract Recent advances in gene editing technologies have transformed the genetic engineering of Chinese hamster ovary (CHO) hosts, enabling the development of cell lines with improved stability and productivity. In this study, we employed the programmable nuclease (PN) Cas‐CLOVER to precisely target the Glutamine synthetase (GS) locus in CHO cells. A
Tiffany McLamarrah +13 more
wiley +1 more source
Biotechnology Progress, EarlyView.This study established the first creation of a commercially available double GS knockout (DKO) from a single KO in CHO‐K1 cells, called CleanCut GS CHO using Cas‐CLOVER. When combined with the Harbor‐IN transposase system, CleanCut GS CHO cells demonstrated a highly antibody productivity.
Cintia Gomez Limia +7 more
wiley +1 more source
Process development for high-titer production of adenovirus devoid of replication-competent particles in suspension-adapted complementing A549 cell culture. [PDF]
BMC BiotechnolShen CF +4 more
europepmc +1 more source
The Effect of Lactic Acid in Association With Steam in Reducing Microorganisms in Soft Wheat
Cereal Chemistry, EarlyView.ABSTRACT Background and Objectives Within the food industry, steam is an effective method of microbial reduction for a variety of products. Previous studies have shown that tempering soft wheat with organic acids, as well as steam application, can potentially reduce wheat's microbial load with minor changes in the functional properties of the flour ...
Shpresa Musa +3 more
wiley +1 more source
Biosynthesis of patchoulol via metabolic engineering the oleaginous red yeast <i>Rhodotorula toruloides</i>. [PDF]
Synth Syst BiotechnolZheng X +7 more
europepmc +1 more source
Evaluating Kernza (Thinopyrum intermedium) as a Co‐Fermentation Feedstock for Bioethanol Production
Cereal Chemistry, EarlyView.ABSTRACT Background Kernza (Thinopyrum intermedium), a perennial grain developed by the Land Institute, presents a promising alternative to conventional ethanol feedstocks due to its deep‐root systems, low fertilizer requirements, and carbon sequestration potential.
Edwin Bonefont, Kurt A. Rosentrater
wiley +1 more source
Enhanced synthesis of 1, 3-medium chain-2-long chain triacylglycerols by engineered Saccharomyces cerevisiae. [PDF]
Bioresour BioprocessPeng Z +5 more
europepmc +1 more source
Self‐Supporting Pt–C60@GO Catalytic Cathodes for Advanced Flexible Li–O2 Batteries
Carbon Energy, EarlyView.A self‐supporting three‐dimensional Pt–C60@GO cathode was developed for a flexible Li–O2 battery, which demonstrates remarkable electrocatalytic activity and electrochemical stability, including a high areal capacity of 3.70 mAh cm−2, a low cell overpotential of 0.48 V, and an excellent cycle stability exceeding 100 cycles.
Zhiguang Zhao +12 more
wiley +1 more source
Long-term adaptive laboratory evolution of Saccharomyces cerevisiae for high-titer lactic acid production at low pH. [PDF]
Microb Cell FactAltvater M +3 more
europepmc +1 more source