Results 191 to 200 of about 17,254 (263)

Iterative hybrid model based optimization of rAAV production

Biotechnology Progress, EarlyView.
Abstract Changes in serotype or genetic payload of recombinant adeno associated virus (rAAVs) gene therapies require adapting the transfection conditions of the upstream HEK293 cultivations. This study adopts an iterative model‐based experiment design approach, where increasing data availability is leveraged to evolve models of different complexity ...
Claudio Müller, Gerald Siegwart, Susanne Heider, Michael Sokolov, Angela Botros, Alexandra Umprecht, Moritz von Stosch, Mariano Nicolas Cruz Bournazou   +7 more
wiley   +1 more source

Chromatographic Determination of Permeability-Relevant Lipophilicity Facilitates Rapid Analysis of Macrocyclic Peptide Scaffolds. [PDF]

J Med Chem
Koch G, Engstrom A, Taechalertpaisarn J, Faris J, Ono S, Naylor MR, Lokey RS.   +6 more
europepmc   +1 more source

Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803

Biotechnology Progress, EarlyView.
Abstract D‐Mannitol production was achieved in freshwater Synechocystis sp. PCC6803 via the heterologous expression of mannitol‐1‐phosphate dehydrogenase (mtlD) and mannitol‐1‐phosphatase (m1p) under control of the strong promoter Ptrc1. However, only 5.54 mg L−1 of mannitol was found extracellularly after 7 days of cultivation, likely due to ...
Wenyang Wu, Wei Du, Klaas J. Hellingwerf, Filipe dos Branco dos Santos   +3 more
wiley   +1 more source

Exploration of the Out-of-Phase Phenomenon in Shake Flasks by CFD Calculations of Volumetric Power Input, k_La Value and Shear Rate at Elevated Viscosity. [PDF]

Biotechnol Bioeng
Dinter C, Gumprecht A, Menze MA, Azizan A, Hansen S, Büchs J.   +5 more
europepmc   +1 more source

N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors

Biotechnology Progress, EarlyView.
Abstract One strategy to enhance the production of biological therapeutics is using transient perfusion in the preculture (N‐1 stage) to seed the production culture (N stage) at ultra‐high cell densities (>10 x 106 viable cells/mL). This very high seeding density improves cell culture performance by shortening the timeline and/or achieving higher final
Lucas Lemire, Sebastian‐Juan Reyes, Yves Durocher, Robert Voyer, Olivier Henry, Phuong Lan Pham   +5 more
wiley   +1 more source

Exploring Yeast's Energy Dynamics: The General Stress Response Lowers Maintenance Energy Requirement. [PDF]

Microb Biotechnol
Temelli N, van den Akker S, Weusthuis RA, Bisschops MMM.   +3 more
europepmc   +1 more source

Comparison of the behavior of CHO cells during cultivation in 24-square deep well microplates and conventional shake flask systems. [PDF]

Biotechnol Rep (Amst), 2014
Chaturvedi K, Sun SY, O'Brien T, Liu YJ, Brooks JW.   +4 more
europepmc   +1 more source

LC‐DAD‐MSn and HR‐LC‐QTOF analysis of Ziziphus budhensis leaves and evaluation of their in vitro and in vivo biological activities

Chemistry &Biodiversity, Accepted Article.
Ziziphus budhensis is a species described in 2015 in Nepal poorly studied for its phytochemical composition and possible bioactivities. In this study, six extracts were obtained from Z. budhensis leaves using ultrasound assisted extraction with solvents in increasing polarity.
Samjhana Bharati, Binita Maharjan, Timila Shrestha, Shyam Sharan Shrestha, Stefania Sut, Hari Prasad Devkota, Ram Lal Swagat Shrestha, Stefano Dall'Acqua   +7 more
wiley   +1 more source

Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B₀. [PDF]

Synth Syst Biotechnol
Zhang X, Cheng S, Yang J, Lu L, Deng Z, Bian G, Liu T.   +6 more
europepmc   +1 more source

Phytochemistry, Antibacterial and Antioxidant Activities of Grewia lasiocarpa E. Mey. Ex Harv. Fungal Endophytes: A Computational and Experimental Validation Study

Chemistry &Biodiversity, EarlyView.
Schematics workflow for the isolation and characterisation of endophytic fungi from grewia lasiocarpa and their biological activity. Abstract The genus Grewia are well‐known for their medicinal properties and are widely used in traditional remedies due to their rich phytochemical composition and potential health benefits.
Nneka Augustina Akwu, Yougasphree Naidoo, Moganavelli Singh, Johnson Lin, Jamiu Olaseni Aribisala, Saheed Sabiu, Makhotso Lekhooa, Adeyemi Oladapo Aremu   +7 more
wiley   +1 more source