Results 71 to 80 of about 505,096 (311)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Preliminary results on the characterization and performances of ZBLAN fiber for infrared spectrographs

open access: yes, 2014
Present telescopes and future extremely large telescopes make use of fiber-fed spectrographs to observe at optical and infrared wavelengths. The use of fibers largely simplifies the interfacing of the spectrograph to the telescope.
Iuzzolino, M.   +4 more
core   +1 more source

Flow-cytometric quantification of microbial cells on sand from water biofilters [PDF]

open access: yes, 2018
Rapid quantification of absolute microbial cell abundances is important for a comprehensive interpretation of microbiome surveys and crucial to support theoretical modelling and the design of engineered systems.
Davenport, Russell J.   +4 more
core   +1 more source

Alcohol‐induced altered glycans in human tracheal epithelial cells promote bacterial adhesion

open access: yesFEBS Open Bio, EarlyView.
Alcohol induces altered glycans to promote bacteria adhesion. Heavy alcohol drinking is known to increase the risk of bacterial pneumonia. However, the link between alcohol levels and risk of infection remains underexplored. Recently, we found that alcohol induced α2‐6sialo mucin O‐glycans in human tracheobronchial epithelial cells, which mediated the ...
Pi‐Wan Cheng   +2 more
wiley   +1 more source

Stability of Dry-Stack Masonry [PDF]

open access: yes, 2006
Student Number : 0100677A - PhD thesis - School of Civil and Environmental Engineering - Faculty of Engineering and the Built EnvironmentThis thesis presents the findings on empirical study of dry-stack masonry.
Ngowi, Joseph Vincent
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Advances in Real-Time Hybrid Testing Technology for Shaking Table Substructure Testing

open access: yesFrontiers in Built Environment, 2020
Shaking table substructure testing (STST) takes the substructure with complex behavior physically tested, with the behavior of the rest structural system being numerically simulated.
Yingpeng Tian   +3 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Experiment program of shake table test on a precast frame made of recycled aggregate concrete

open access: yesHo Chi Minh City Open University Journal of Science - Engineering and Technology, 2016
A precast frame model made of Recycled Aggregate Concrete (RAC) been constructed with precast beams, columns and Cast-In-Place (CIP) joints. Then a shaking table test was carried out with three types of earthquake ground motions, namely Wenchuan, El ...
Pham Thi Loan, Phan Van Hue
doaj  

Dynamic Characteristics of Metro Tunnel Closely Parallel to a Ground Fissure

open access: yesComplexity, 2019
Shaking table model test and numerical simulation model were conducted on scaled tunnel model to investigate the mechanism and effect of seismic loadings on metro tunnel which is closely parallel to an active ground fissure.
Nina Liu   +6 more
doaj   +1 more source

Home - About - Disclaimer - Privacy