Results 71 to 80 of about 8,220,657 (249)

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Advances in Real-Time Hybrid Testing Technology for Shaking Table Substructure Testing

Frontiers in Built Environment, 2020
Shaking table substructure testing (STST) takes the substructure with complex behavior physically tested, with the behavior of the rest structural system being numerically simulated.
Yingpeng Tian, Xiaoyun Shao, Huimeng Zhou, Tao Wang   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Dynamic Characteristics of Metro Tunnel Closely Parallel to a Ground Fissure

Complexity, 2019
Shaking table model test and numerical simulation model were conducted on scaled tunnel model to investigate the mechanism and effect of seismic loadings on metro tunnel which is closely parallel to an active ground fissure.
Nina Liu, Quanzhong Lu, Xiaoyang Feng, Wen Fan, Jianbing Peng, Weiliang Liu, Xin Kang   +6 more
doaj   +1 more source

Design on the Shaking Table Test for Ground Crack Dynamic Response under Earthquake

Chemical Engineering Transactions, 2016
In this paper, according to a particular ground fissures in Xi’an city in the south of the actual stratigraphic profiles use a similar role of strong earthquakes and the theory of vibration table crack mechanical response model test for similar relation ...
M.M. Liu, F.F. Dong
doaj   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

Dynamic Failure Experimental Study of a Gravity Dam Model on a Shaking Table and Analysis of Its Structural Dynamic Characteristics

Sensors
Investigating the dynamic response patterns and failure modes of concrete gravity dams subjected to strong earthquakes is a pivotal area of research for addressing seismic safety concerns associated with gravity dam structures.
Jianchun Qiu, Wenqin He, Dongjian Zheng, Yanxin Xu, Shaolong Guo, Tianxiao Ma, Pengcheng Xu, Yongtao Liu   +7 more
doaj   +1 more source

Seismic response of micropiles-reinforced landslide based on shaking table test

Geomatics, Natural Hazards & Risk, 2019
A series of shaking table tests were conducted on unreinforced and micropiles-reinforced soil landslides to investigate the seismic response of micropiles-reinforced landslide and the dynamic mechanical behaviour of micropiles in landslide under ...
Nan Li, Yuming Men, Liqun Yuan, Banqiao Wang, Jun Li, Xueling Liu   +5 more
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source