Results 41 to 50 of about 2,427,049 (304)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Column bases in shear and normal force [PDF]
, 2008 Connections of steel columns to concrete foundations may be loaded by combinations of normal force, bending moment and shear force. Shear force will primarily be transmitted by friction between the base plate and the grout layer to the concrete ...
Wald, F. (author) +7 more
core +1 more source
Advanced Intelligent SystemsFlexible force sensors have been widely applied in robotics for object exploring, remote controlling, and human–machine interactions. The tri‐axis force sensors for accurate measurement of normal and shear forces are generally in urgent demand.
Jie Jin +4 more
doaj +1 more source
Shearing mechanical model and experimental verification of bolts in jointed rock mass
Yantu gongcheng xuebao, 2018 Based on the principle of the minimum residual energy, the relationship between the horizontal shear displacement of the joint surface and the axial and tangential deformation of the bolt is analyzed considering the shear deformation of the bolts in ...
LIU Quan-sheng 1, 2, LEI Guang-feng 1,3, , PENG Xing-xin 1, 3, WEI Lai 1, 3
doaj +1 more source
Optimization the Resistance Spot Welding Parameters of Austenitic Stainless Steel and Aluminum Alloy Using Design of Experiment Method [PDF]
Engineering and Technology Journal, 2016 This research aims to study the effect of RSW parameters on the sheerforce of the spot weldedfor two materials {AISI 304L and AA 6061-T6}with (0.5 and 0.7 mm) thickness.
Sabah Khammass Hussein +1 more
doaj +1 more source
Universal anisotropy in force networks under shear [PDF]
Physical Review E, 2007 4+ pages, 3 ...
Ostojic, S., Vlugt, T.J.H., Nienhuis, B.
openaire +6 more sources
Hydrogel platforms for 3D cardiac tissue engineering and fibrotic disease modelling
FEBS Open Bio, EarlyView.Hydrogel‐based cardiac tissue models are advancing our understanding of heart health and disease. By incorporating electrical conductivity, aligned structures, and tunable mechanical properties, these platforms better replicate the complex environment of native heart tissue.
Harrison Porritt +2 more
wiley +1 more source
Arthritis Care &Research, EarlyView.Systemic sclerosis (SSc) is a rare autoimmune disease defined by immune dysregulation, vasculopathy, and progressive fibrosis of the skin and internal organs. Despite advances in care, major complications such as interstitial lung disease (ILD) and myocardial involvement remain the leading causes of morbidity and mortality.
Cristiana Sieiro Santos +2 more
wiley +1 more source