Results 211 to 220 of about 4,956,823 (296)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Impact of physician shift work implementation on mortality and length of stay in an emergency and critical care center: an interrupted time series analysis. [PDF]

BMC Health Serv Res
Nakae R, Takiguchi T, Igarashi Y, Hirano T, Hamaguchi T, Tominaga N, Shigeta K, Yokobori S.   +7 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Health Implications of Shift Work in Airline Pilots and Cabin Crew: A Narrative Review and Pilot Study Findings. [PDF]

Nutrients
Stefańska O, Barbarska O, Minkiewicz-Zochniak A.   +2 more
europepmc   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Socioeconomic determinants of shift work employment in Canada and the Netherlands: a cross-sectional analysis of data from the Canadian Longitudinal Study on Aging and the Hoorn Study. [PDF]

Front Public Health
Campbell DB, Reed T, Slebe R, Raina P, Rutters F, Joshi D, de Mutsert R, Despres JP, Hoeks J, Blondin DP, Carpentier A, Schrauwen P, Campbell DJT.   +12 more
europepmc   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source

Organizational Silence and Related Factors Among Shift Work Nurses in Korea: A Cross-Sectional Study. [PDF]

J Nurs Manag
Eon Sim S, Jang HY.
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Guidelines for Reducing the Adverse Effects of Shift Work on Nursing Staff: A Systematic Review. [PDF]

Healthcare (Basel)
Inchingolo AD, Inchingolo AM, Fatone MC, Ferrante L, Casamassima L, Trilli I, Inchingolo F, Palermo A, Marinelli G, Dipalma G.   +9 more
europepmc   +1 more source