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Transforming Shiga toxin-producing Escherichia coli surveillance through whole genome sequencing in food safety practices

open access: yesFrontiers in Microbiology, 2023
IntroductionShiga toxin-producing Escherichia coli (STEC) is a gastrointestinal pathogen causing foodborne outbreaks. Whole Genome Sequencing (WGS) in STEC surveillance holds promise in outbreak prevention and confinement, in broadening STEC epidemiology
Stéphanie Nouws   +11 more
doaj   +1 more source

Comparison of Two Shiga Toxin-producing Escherichia coli (STEC) Isolation Protocols in Raw Cow’s Milk Cheese Enrichment Broths: Direct STEC Isolation Versus Techniques Based on Immuno-concentration

open access: yesJournal of Food Protection, 2023
The presence of Shiga toxin-producing Escherichia coli (STEC) in dairy products made with raw milk is a major concern for food safety authorities and industries. Two approaches have been proposed to isolate STEC from food.
Stephane D. Miszczycha   +3 more
doaj   +1 more source

Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies

open access: yesFrontiers in Microbiology, 2021
The current routine laboratory practices to investigate food samples in case of foodborne outbreaks still rely on attempts to isolate the pathogen in order to characterize it.
Florence E. Buytaers   +11 more
doaj   +1 more source

Surface proteins of Shiga toxin-producing Escherichia coli mediate association with milk fat globules in raw milk

open access: yesFrontiers in Microbiology, 2023
IntroductionBy adhering to host cells and colonizing tissues, bacterial pathogens can successfully establish infection. Adhesion is considered the first step of the infection process and bacterial adhesion to anti-adhesive compounds is now seen as a ...
Arthur Bagel   +10 more
doaj   +1 more source

Volume Resuscitation and Progression to Organ Failure in Shiga Toxin-Producing Escherichia coli Infection in Adults

open access: yesCritical Care Explorations, 2021
OBJECTIVES:. Shiga toxin-producing Escherichia coli infection is associated with dysentery and the hemolytic uremic syndrome, marked by the triad of microangiopathic hemolytic anemia, acute kidney failure, and thrombocytopenia.
Charles G. Volk, MD   +4 more
doaj   +1 more source

A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine

open access: yesMicroorganisms, 2020
The management of a foodborne outbreak depends on the rapid and accurate identification of the responsible food source. Conventional methods based on isolation of the pathogen from the food matrix and target-specific real-time polymerase chain reactions (
Florence E. Buytaers   +8 more
doaj   +1 more source

The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country

open access: yesFoods, 2020
Gradually, conventional methods for foodborne pathogen typing are replaced by whole genome sequencing (WGS). Despite studies describing the overall benefits, National Reference Laboratories of smaller countries often show slower uptake of WGS, mainly ...
Stéphanie Nouws   +10 more
doaj   +1 more source

Hemolytic uremic syndrome related to Shiga-like toxin-producing Escherichia coli with encephalitis hiding a human herpesvirus-6 infection: a case report

open access: yesJournal of Medical Case Reports, 2021
Background Cardiac and neurological involvement in hemolytic uremic syndrome are life-threatening complications. The most frequent complications of cardiac involvement in hemolytic uremic syndrome are myocarditis and cardiac dysfunction due to fluid ...
Sophie Mounier   +5 more
doaj   +1 more source

A brief overview of emergencies and dissemination of Shiga-toxin-producing E. coli (STEC) and Salmonella enterica serovar Typhimurium DT104 in humans and food producing animals

open access: yesArchives of Veterinary Medicine, 2020
Shiga-toxin-producing Escherichia coli (STEC) and Salmonella enterica serovar Typhimurium (STDT104) are foodborne pathogens of public health significance.
Maja Velhner   +6 more
doaj   +1 more source

Heparin-induced thrombocytopenia following Shiga-toxin-associated hemolytic uremic syndrome: a case report

open access: yesJournal of Medical Case Reports, 2022
Background Up to 50% of cases of Shiga-toxin-producing Escherichia coli hemolytic uremic syndrome occur in adults, and the clinical presentation is variable.
Elia Rigamonti   +3 more
doaj   +1 more source

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