Results 181 to 190 of about 19,735 (216)

Protection of Monkeys against Shiga Toxin Induced by Shiga Toxin-Liposome Conjugates

International Archives of Allergy and Immunology, 2002
<i>Background:</i> We previously reported that the purified Shiga toxins (Stx) Stx1 and Stx2, when coupled with liposomes, induced substantial production of anti-Stx1 and anti-Stx2 IgG antibody, respectively, in mice. The levels of anti-Stx antibody in the sera of mice immune to Stx-liposome correlated well with the protection against ...
Yuriko, Suzaki, Yasushi, Ami, Noriyo, Nagata, Seishiro, Naito, Hiroshi, Kato, Maiko, Taneichi, Motohide, Takahashi, Takako, Komiya, Sachihiro, Satoh, Fumio, Gondaira, Junichi, Sugiyama, Yoshio, Nakano, Masahito, Mori, Katsutoshi, Komuro, Tetsuya, Uchida   +14 more
openaire   +2 more sources

Shiga-toxin-converting bacteriophages

Research in Microbiology, 2001
Shiga toxins (Stx) comprise a family of potent cytotoxins that are involved in severe human disease. Stx are mainly produced by Escherichia coli isolated from human and nonhuman sources, and by Shigella dysenteriae type 1. The genes encoding Stx are thought to be generally encoded in the genome of lambdoid prophages (Stx-converting bacteriophages; Stx ...
openaire   +2 more sources

Human Milk Contains the Shiga Toxin and Shiga-like Toxin Receptor Glycolipid Gb3

The Journal of Infectious Diseases, 1992
Human milk antibody and nonantibody factors are thought to be important in protecting infants from diarrheal diseases. The nonantibody factors include host receptor analogues that bind to specific pathogen virulence factors, thereby inhibiting these bacterial products from binding to their intestinal target receptors.
D S, Newburg, S, Ashkenazi, T G, Cleary
openaire   +2 more sources

Shiga Toxin-Producing Escherichia coli

2014
In the United States, it is estimated that non-O157 Shiga toxin-producing Escherichia coli (STEC) cause more illnesses than STEC O157:H7, and the majority of cases of non-O157 STEC infections are due to serogroups O26, O45, O103, O111, O121, and O145, referred to as the top six non-O157 STEC.
James L, Smith, Pina M, Fratamico, Nereus W, Gunther   +2 more
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Shiga Toxin: Purification, Structure, and Function

Clinical Infectious Diseases, 1991
Shiga toxin is a potent toxin produced by Shigella dysenteriae type 1 strains. The toxin has three biologic activities--cytotoxicity, enterotoxicity, and neurotoxicity--and one known biochemical effect: inhibition of protein synthesis. It consists of two polypeptide chains, an A chain (molecular weight, 32,225) and a B chain (molecular weight, 7,691 ...
A, Donohue-Rolfe, D W, Acheson, G T, Keusch   +2 more
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Detection Methods for Shiga Toxins and Shiga Toxin-Producing E. coli

2017
Shiga toxin detection methods reflect the complicated biological processes that deliver these toxins to their target cells. Some detection methods employ techniques that indirectly detect the presence of Shiga toxins. Microbiological methods are used to identify pathogenic serotypes of E. coli, such as the infamous O157:H7, but the potential production
Christopher J. Silva, David L. Brandon, Craig B. Skinner, Xiaohua He   +3 more
openaire   +1 more source

Entry of Shiga Toxin into Cells

Zentralblatt für Bakteriologie, 1993
The effect of Shiga toxin with mutations in the A fragment has been tested on cells in order to get more information about the processing of the A fragment during entry into the cytosol. A mutant with a deletion between the A1 and A2 domain in the A fragment is resistant to cleavage by trypsin and is less toxic than wild type toxin on both Vero and ...
Sandvig, Kirsten, Dubinina, Elena, Garred, Øystein, Prydz, Kristian, Kozlov, Juri V., Hansen, Steen H., van Deurs, Bo   +6 more
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Phage display and Shiga toxin neutralizers

Toxicon, 2016
The current work presents an overview of the use of phage display technology for the identification and characterization of potential neutralizing agents for Shiga toxins. The last major Shiga toxin-associated disease outbreak, which took place in Germany in 2011, showed the international community that Shiga toxins remain a serious threat to public ...
Robert Alvin, Bernedo-Navarro, Tomomasa, Yano   +1 more
openaire   +2 more sources

Affinity purification of Shiga-like toxin I and Shiga-like toxin II

Journal of Microbiological Methods, 1998
Abstract A facile method is described for affinity purifying Shiga-like toxin I (verotoxin 1) or Shiga-like toxin II (verotoxin 2) from cell-free Escherichia coli culture supernatant solutions using immobilized synthetic analogs of the digalactoside [αGal(1,4)βGal] host cell receptor for these toxins.
George Mulvey, Rosa Vanmaele, Monique Mrazek, Mark Cahill, Glen D. Armstrong   +4 more
openaire   +1 more source

Human Milk Lipids Bind Shiga Toxin

2001
Hemolytic uremic syndrome, a serious complication of Shiga toxin-associated diarrhea, is rare before 6 months of age. Immunologic and nonimmunologic factors present in human milk may partially explain this observation. In prior studies, we have demonstrated that human milk contains Gb3, the receptor for the B subunit of Shiga toxin, and also contains ...
I, Herrera-Insua, H F, Gomez, V A, Diaz-Gonzalez, P, Chaturvedi, D S, Newburg, T G, Cleary   +5 more
openaire   +2 more sources