Results 41 to 50 of about 389,510 (254)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Optimisation of the bone single photon emission computed tomography in oncology patients

Journal of Health Sciences
Introduction: The introduction of hybrid imaging systems such as Single Photon Emission Computed Tomography/Computed Tomography (SPECT/CT) has completely changed the scanning procedure of conventional diagnostic nuclear medicine protocols.
Nusret Salkica, Amela Begić, Adnan Beganović, Fuad Julardžija, Adnan Šehić, Halil Ćorović, Safet Hadžimusić, Amra Skopljak-Beganović   +7 more
doaj   +1 more source

Measuring the signal-to-noise ratio of a neuron [PDF]

Proceedings of the National Academy of Sciences, 2015
Significance Neurons represent both signal and noise in binary electrical discharges termed action potentials. Hence, the standard signal-to-noise ratio (SNR) definition of signal amplitude squared and divided by the noise variance does not apply. We show that the SNR estimates a ratio of expected prediction errors.
Czanner, Gabriela, Sarma, Sridevi V., Ba, Demba, Eden, Uri T., Wu, Wei, Eskandar, Emad N., Lim, Hubert H., Temereanca, Simona, Suzuki, Wendy A., Brown, Emery N.   +9 more
openaire   +4 more sources

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Material imaging study of 3D printing materials for diagnostic radiology phantom development

Brazilian Journal of Radiation Sciences
The 3D printing techniques have found applications across diverse fields, significantly enhancing design and manufacturing processes. The impact of this growth is particularly notable in radiology, where 3D printing has been applied to developing quality
Marcus Oliveira, Matheus Savi, Adriano Vitor, Daniel Villani, Marco Andrade, Carlos Ubeda, Mauricio Mitsuo Monção   +6 more
doaj   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

3D MR neurography with gadolinium contrast to improve the visualization of pelvic nerves and the branches

Frontiers in Physiology
ObjectiveTo evaluate the effectiveness of 3D NerveVIEW sequence with gadolinium contrast on the visualization of pelvic nerves and their branches compared to that without contrast.MethodsParticipants were scanned twice using 3D NerveVIEW sequence with ...
Hui Liu, Zhibo Xiao, Li Tao, Mingmei Tang, Yong Xu, Yuanrui Pan, Ke Zhang, Xueke Qiu, Fajin Lv, Fajin Lv   +9 more
doaj   +1 more source