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Silver Staining in Clinical Cytogenetics
Stain Technology, 1985Silver staining of human chromosomes at prometaphase or metaphase identifies variants in the stalk (nucleolar organizing) regions of acrocentric chromosomes (Nos. 13, 14, 15, 21, 22). Variants are defined by size, number, and morphology of silver staining areas.
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Silver Staining Techniques of Polyacrylamide Gels
2012After SDS-polyacrylamide gel electrophoresis the separated proteins have to be visualized by staining the gel. The same is true after transfer of separated proteins to a blotting membrane in order to verify an efficient transfer and to visualize the amount of protein(s) remaining in the gel.
Berndt, N. +4 more
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Chemical studies on the silver staining of nucleoli
Experientia, 1980The uptake of Ag-ions by isolated nucleoli of rat liver cells was studied. Nucleolar proteins separated by electrophoresis were examined for selective silver-staining. A mechanism for preferential Ag-staining of the nucleoli is discussed.
H, Kling +4 more
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Silver staining of proteins in polyacrylamide gels
Analytical Biochemistry, 1981Abstract The silver-staining procedure for detecting proteins in polyacrylamide gels has been modified and further simplified so that it is stable, controllable, and even more rapid than previous silver-staining methods. The method retains its sensitivity to proteins at the nanogram level and may be used either before or after Coomassie blue staining.
W, Wray +3 more
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A Simple Silver Solution for Staining Reticulin
Stain Technology, 1974A method for staining reticulin in formalin fixed, paraffin embedded sections is described. The steps include oxidation in permangauate, impregnation in a silver solution, reduction in formalin and toning in gold. The permanganate is acidified, enhancing its oxidizing potency and decreasing the stainiig of background elements.
J, Naoumenko, I, Feigin
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A silver staining reaction for chromosomes and nuclei
Histochemie, 1965A staining reaction for the nucleus and chromosomes, using silver-hexamethylentetramine, buffered with borax, after hydrolisis with warm IN HC1, has been proposed. Its specificity has been assayed by means of enzymatic digestions (DNase, pepsin, trypsin), and through the comparison of the results with those obtained by the Feulgen reaction.
C, De Martino +3 more
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Mitochondrial nucleoid staining with ammoniacal silver
Experimental Cell Research, 1974Abstract Under controlled conditions, ammoniacal silver (A-S) reaction appears in the nucleoid in the mitochondria of Physarum polycephalum as well as the dense chromatin in the nuclei. The results and the effect of selective extraction of basic proteins on the A-S reaction of the nucleoid and the nucleus suggest that the mitochondrial nucleoid may ...
T, Kuroiwa, M, Hizume
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Selective Silver Staining for Neural Degeneration
2003Silver degeneration stains are typically considered the domain of studies of neural circuitry, but have also proven useful in studies of neurotoxicity. They are particularly well suited to localizing sites of injury, and determining the extent and time-course of degeneration.
J K, Olin, K F, Jensen
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Silver staining of NORs in electron microscopy
Experimental Cell Research, 1979Abstract We adapted the Ag-As procedure for staining the nucleolus organizer regions (NORs) to the ultrastructural level, under conditions preserving the specificity of the method. The fibrillar centre and the dense fibrillar component appeared to constitute the interphasic counterpart of the NOR plus the RNA transcription sites.
C A, Bourgeois +3 more
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The silver staining of reticular fibers by electrophoresis
The Anatomical Record, 1965AbstractIn this technique reticulin is stained black with silver by the electrophoretic method. Use Zenker or formalin (10%) fixed tissue, but mordant the latter in Zenker stock for one hour or longer‥ Carry paraffin sections to water; mordant, if necessary; wash well in tap water; transfer to distilled water; place slide in tray containing the ...
C W, MONROE, R G, HARRIS
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