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Sindbis Virus: Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2005
AbstractThe prototype of the Alphaviruses, Sindbis virus, has a broad host range. In nature, Sindbis virus shuttles from an insect vector to a vertebrate host and back to the insect vector in a complex transmission cycle. Sindbis virus must, therefore, be able to replicate in two biochemically and genetically divergent hosts, invertebrates and ...
Christine Sinodis   +2 more
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The origin of the protein of sindbis virus

Virology, 1964
Abstract Tyrosine was shown to be an essential amino acid for protein synthesis in primary chick fibroblast tissue cultures. When C14- l -tyrosine was added to the tissue cultures at the time of high-multiplicity infection with Sindbis virus, the specific activities of tyrosine in the hydrolyzate of viral protein and in the medium were similar. Cells
E.R. Pfefferkorn, Ruth L. Clifford
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On the infectivity of the Sindbis virus nucleocapsid

Canadian Journal of Microbiology, 1970
The nucleocapsid fraction derived either from Sindbis virions or from infected cell cultures was studied with respect to its ability to adsorb to susceptible cells as well as to initiate stages in viral replication. Neither preparation was adsorbed to BHK 21 cells under conditions where virion adsorption could be demonstrated. In addition parental RNA
P. Dobos, P. Faulkner
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Semliki Forest Virus and Sindbis Virus Vectors

Current Protocols in Human Genetics, 2002
AbstractSemliki Forest virus (SFV) and Sindbis virus (SIN) are two, positive‐strand RNA viruses of the alphavirus genus. Vectors for both have been developed to express high levels of foreign genes in vitro and in vivo. describes the preparation of packaged SFV and SIN replicons by co‐electroporation of helper and vector RNA into baby hamster kidney ...
Markus U. Ehrengruber, Kenneth Lundstrom
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Glycosylation of intracellular Sindbis virus glycoproteins

Biochemistry, 1982
Oligosaccharides of purified intracellular Sindbis virus glycoproteins have been examined by high-resolution Bio-Gel chromatography. The array of glycopeptides from cellular E1 and E2 appeared similar to the glycopeptides (S1, S2, S3, and S4) found in mature virus glycoproteins described previously [Hakimi, J., & Atkinson, P. H. (1980) Biochemistry 19,
John Hakimi, Paul H. Atkinson
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Sindbis Virus: A Newly Recognized Arthropod-Transmitted Virus

The American Journal of Tropical Medicine and Hygiene, 1955
Summary 1.  Studies on Sindbis virus, a newly recognized member of the arthropod-transmitted viruses, are described; these include data on its immunological characteristics, distribution in the Nile Valley, vertebrate host range, and transmissibility by arthropods. 2.
Herbert S. Hurlbut   +4 more
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Lipid composition of Sindbis virus

Virology, 1971
Abstract The phospholipid and fatty acid compositions of Sindbis virus prepared from chick embryo fibroblasts and BHK cells were found to be very similar. These were compared to the compositions of the whole cells and plasma membranes of the two hosts. It was found that the viral compositions were distinct from the cellular compositions in both cases
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Immune lysis of Sindbis virus

Virology, 1975
Abstract A simple technique is reported for the demonstration of immune lysis of Sindbis virus. The reagents required are virus labeled in its RNA, antiviral antibody, complement, and ribonuclease. The method is considerably simpler than those previously used to demonstrate immune virolysis.
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