Results 71 to 80 of about 10,820,161 (256)

Small RNA transcriptome analysis using parallel single-cell small RNA sequencing

Scientific Reports, 2023
miRNA and other forms of small RNAs are known to regulate many biological processes. Single-cell small RNA sequencing can be used to profile small RNAs of individual cells; however, limitations of efficiency and scale prevent its widespread application ...
Jia Li, Zhirong Zhang, Yinghua Zhuang, Fengchao Wang, Tao Cai   +4 more
doaj   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing [PDF]

, 2020
This protocol presents a plate-based workflow to perform RNA sequencing analysis of single cells/nuclei using Smart-seq2. We describe (1) the dissociation procedures for cell/nucleus isolation from the mouse brain and human organoids, (2) the flow ...
Jaeger, Baptiste N., Kruse, Merit, Jaeger, Baptiste N, Yángüez, Emilio, Karayannis, Theofanis, Denoth-Lippuner, Annina, Jessberger, Sebastian, Karayannis, Theofanis; https://orcid.org/, Gesuita, Lorenzo   +8 more
core   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

Simulating multiple faceted variability in single cell RNA sequencing

Nature Communications, 2019
Simulated single cell RNA sequencing data is useful for method development and comparison. Here, the authors developed SymSim, a simulator that explicitly models the main factors of variation in single cell data.
Xiuwei Zhang, Chenling Xu, Nir Yosef
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Splatter: simulation of single-cell RNA sequencing data

Genome Biology, 2017
As single-cell RNA sequencing (scRNA-seq) technologies have rapidly developed, so have analysis methods. Many methods have been tested, developed, and validated using simulated datasets.
Luke Zappia, Belinda Phipson, Alicia Oshlack   +2 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

Single-cell RNA sequencing of human kidney

, 2019
A comprehensive cellular anatomy of normal human kidney is crucial to address the cellular origins of renal disease and renal cancer. Some kidney diseases may be cell type-specific, especially renal tubular cells.
Zhenyuan Yu (6698111), Jinling Liao (7312032), Yang Chen (7312037)   +2 more
core   +1 more source

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Biosensors
Single-cell RNA sequencing is a high-throughput novel method that provides transcriptional profiling of individual cells within biological samples.
Roozbeh Abedini-Nassab, Fatemeh Taheri, Ali Emamgholizadeh, Hossein Naderi-Manesh   +3 more
doaj   +1 more source