Results 51 to 60 of about 734,534 (263)

Single-cell transcriptome analysis for cancer and biology of the pancreas: A review on recent progress

Frontiers in Genetics, 2023
Single-cell sequencing has become one of the most used techniques across the wide field of biology. It has enabled researchers to investigate the whole transcriptome at the cellular level across tissues, which unlocks numerous potentials for basic and ...
Mona Tamaddon, Mostafa Azimzadeh, Mostafa Azimzadeh, Mostafa Azimzadeh, Peyman Gifani, Peyman Gifani, Seyed Mohammad Tavangar, Seyed Mohammad Tavangar   +7 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing [PDF]

, 2020
This protocol presents a plate-based workflow to perform RNA sequencing analysis of single cells/nuclei using Smart-seq2. We describe (1) the dissociation procedures for cell/nucleus isolation from the mouse brain and human organoids, (2) the flow ...
Jaeger, Baptiste N., Kruse, Merit, Jaeger, Baptiste N, Yángüez, Emilio, Karayannis, Theofanis, Denoth-Lippuner, Annina, Jessberger, Sebastian, Karayannis, Theofanis; https://orcid.org/, Gesuita, Lorenzo   +8 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Deep sequence analysis of non-small cell lung cancer: Integrated analysis of gene expression, alternative splicing, and single nucleotide variations in lung adenocarcinomas with and without oncogenic KRAS mutations [PDF]

, 2012
KRAS mutations are highly prevalent in non-small cell lung cancer (NSCLC), and tumors harboring these mutations tend to be aggressive and resistant to chemotherapy.
David Rossell, Xiaojia eTang, Tang, Xiaojia, Hossain, Asif, Rossell, David, Asha Nair, Jean-Pierre Kocher, Chai, High-Seng, Michael P. Walsh, Asif eHossain, Yan W. Asmann, Baker, Tiffany R., Edith A. Perez, Kalari, Krishna R., Necela, Brian M., Alan P. Fields, High Seng eChai, Xiaojia Tang, Alan P Fields, Leontovich, Alexey A., Carr, Jennifer, Thompson, E. Aubrey, Saurabh Baheti, Kocher, Jean-Pierre, Tiffany R Baker, Younkin, Curtis S., Walsh, Michael P., E. Aubrey Thompson, Jennifer M. Kachergus, Jennifer M Kachergus, Jennifer M Carr, Fields, Alan P., Asmann, Yan W., Krishna R. Kalari, E. Aubrey eThompson, Kachergus, Jennifer M., Steven N Hart, Krishna R Kalari, Jennifer M. Carr, David eRossell, Saurabh eBaheti, Steven N. Hart, Curtis S. Younkin, Zhifu eSun, Edith A Perez, Tiffany Baker, Brian M Necela, Alexey A. Leontovich, Nair, Asha, Perez, Edith A., Sun, Zhifu, Reisman, David N., Jean-Pierre eKocher, Asha eNair, Hart, Steven N., Yan W Asmann, Zhifu Sun, Michael eWalsh, Baheti, Saurabh, David N Reisman, Brian M. Necela, High-Seng Chai, David N. Reisman, Alexey A Leontovich, Asif Hossain, Curtis S Younkin   +65 more
core   +1 more source

Gene length is a pivotal feature to explain disparities in transcript capture between single transcriptome techniques

Frontiers in Bioinformatics, 2023
The scale and capability of single-cell and single-nucleus RNA-sequencing technologies are rapidly growing, enabling key discoveries and large-scale cell mapping operations.
Ricardo R. Pavan, Fabiola Diniz, Samir El-Dahr, Giovane G. Tortelote   +3 more
doaj   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Clustering of single-cell RNA sequencing datasets with the hierarchical Dirichlet process [PDF]

Single-cell sequencing is a powerful technology that allows researchers to understand and analyse genetic and genomic information of individual cells within a heterogeneous population. This thesis focuses on two types of single-cell data, namely, 1) high-
Liu, Jinlu
core   +1 more source

Single-cell transcriptome sequencing: recent advances and remaining challenges [version 1; referees: 2 approved]

F1000Research, 2016
Single-cell RNA-sequencing methods are now robust and economically practical and are becoming a powerful tool for high-throughput, high-resolution transcriptomic analysis of cell states and dynamics.
Serena Liu, Cole Trapnell
doaj   +1 more source

Automatic cell type identification methods for single-cell RNA sequencing

Computational and Structural Biotechnology Journal, 2021
Single-cell RNA sequencing (scRNA-seq) has become a powerful tool for scientists of many research disciplines due to its ability to elucidate the heterogeneous and complex cell-type compositions of different tissues and cell populations. Traditional cell-
Bingbing Xie, Qin Jiang, Antonio Mora, Xuri Li   +3 more
doaj   +1 more source