Results 41 to 50 of about 10,820,161 (256)

Comparative Analysis of Single-cell and Single-nucleus RNA-sequencing in a Rabbit Model of Retinal Detachment-related Proliferative Vitreoretinopathy

Ophthalmology Science, 2023
Purpose: Proliferative vitreoretinopathy (PVR) is the most common cause of failure of retinal reattachment surgery, and the molecular changes leading to this aberrant wound healing process are currently unknown.
Clayton P. Santiago, PhD, MS, Megan Y. Gimmen, BSc, Yuchen Lu, BSc, Minda M. McNally, MS, Leighton H. Duncan, MA, BSc, Tyler J. Creamer, PhD, Linda D. Orzolek, MS, Seth Blackshaw, PhD, Mandeep S. Singh, MD, PhD   +8 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

CRISPR screening in single cells

, 2019
The combination of single-cell RNA-seq and CRISPR allows for efficient interrogation of possibly any number of genes, only limited by the sequencing capability. Here we describe the current protocols for CRISPR screening in single cells, from cloning and
Henriksson, Johan,
core   +1 more source

Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing [PDF]

, 2017
Summary: The gonad is a unique biological system for studying cell-fate decisions. However, major questions remain regarding the identity of somatic progenitor cells and the transcriptional events driving cell differentiation.
Emmanouil T. Dermitzakis, Serge Nef, Antonarakis, Stylianos E., Neirijnck, Yasmine, Nef, S, Yasmine Neirjinck, Isabelle Stévant, Neirijnck, Y, Jessica Escoffier, Stévant, I, Borel, Christelle, Dermitzakis, ET, Stévant, Isabelle, Nef, Serge, Smith, Lee B., Dermitzakis, Emmanouil T, Lee B. Smith, Escoffier Breancon, Jessica, Borel, C, Antonarakis, Stylianos, Stylianos E. Antonarakis, Antonarakis, SE, Dermitzakis, Emmanouil, Escoffier, J, Smith, LB, Christelle Borel, Smith, Lee B, Escoffier, Jessica, Yasmine Neirijnck   +28 more
core   +1 more source

Single-Cell RNA-Sequencing: Assessment of Differential Expression Analysis Methods

Frontiers in Genetics, 2017
The sequencing of the transcriptomes of single-cells, or single-cell RNA-sequencing, has now become the dominant technology for the identification of novel cell types and for the study of stochastic gene expression.
Alessandra Dal Molin, Giacomo Baruzzo, Barbara Di Camillo   +2 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Deep sequence analysis of non-small cell lung cancer: Integrated analysis of gene expression, alternative splicing, and single nucleotide variations in lung adenocarcinomas with and without oncogenic KRAS mutations [PDF]

, 2012
KRAS mutations are highly prevalent in non-small cell lung cancer (NSCLC), and tumors harboring these mutations tend to be aggressive and resistant to chemotherapy.
David Rossell, Xiaojia eTang, Tang, Xiaojia, Hossain, Asif, Rossell, David, Asha Nair, Jean-Pierre Kocher, Chai, High-Seng, Michael P. Walsh, Asif eHossain, Yan W. Asmann, Baker, Tiffany R., Edith A. Perez, Kalari, Krishna R., Necela, Brian M., Alan P. Fields, High Seng eChai, Xiaojia Tang, Alan P Fields, Leontovich, Alexey A., Carr, Jennifer, Thompson, E. Aubrey, Saurabh Baheti, Kocher, Jean-Pierre, Tiffany R Baker, Younkin, Curtis S., Walsh, Michael P., E. Aubrey Thompson, Jennifer M. Kachergus, Jennifer M Kachergus, Jennifer M Carr, Fields, Alan P., Asmann, Yan W., Krishna R. Kalari, E. Aubrey eThompson, Kachergus, Jennifer M., Steven N Hart, Krishna R Kalari, Jennifer M. Carr, David eRossell, Saurabh eBaheti, Steven N. Hart, Curtis S. Younkin, Zhifu eSun, Edith A Perez, Tiffany Baker, Brian M Necela, Alexey A. Leontovich, Nair, Asha, Perez, Edith A., Sun, Zhifu, Reisman, David N., Jean-Pierre eKocher, Asha eNair, Hart, Steven N., Yan W Asmann, Zhifu Sun, Michael eWalsh, Baheti, Saurabh, David N Reisman, Brian M. Necela, High-Seng Chai, David N. Reisman, Alexey A Leontovich, Asif Hossain, Curtis S Younkin   +65 more
core   +1 more source

FEATS: feature selection-based clustering of single-cell RNA-seq data

, 2021
MOTIVATION: Advances in next-generation sequencing have made it possible to carry out transcriptomic studies at single-cell resolution and generate vast amounts of single-cell RNA sequencing (RNA-seq) data rapidly.
Vans, E, Patil, A, Sharma, A
core   +1 more source

Mesothelioma cell heterogeneity identified by single cell RNA sequencing

Scientific Reports
Mesothelioma cell heterogeneity encompasses diverse morphological and molecular characteristics observed within tumors, significantly impacting disease progression, treatment outcomes, and the development of targeted therapies.
Licun Wu, Zongjie Wang, Amin Zia, Shana O. Kelley, Marc de Perrot   +4 more
doaj   +1 more source