SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology [PDF]
, 2018 We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq).
Abdelmoez Mahmoud N. +9 more
core +2 more sources
Pumping the brakes on RNA velocity by understanding and interpreting RNA velocity estimates
Genome Biology, 2023 Background RNA velocity analysis of single cells offers the potential to predict temporal dynamics from gene expression. In many systems, RNA velocity has been observed to produce a vector field that qualitatively reflects known features of the system ...
Shijie C. Zheng +4 more
doaj +1 more source
Single cell RNA sequencing research in maternal fetal interface
Frontiers in Cell and Developmental Biology, 2023 The maternal-fetal interface is an essential environment for embryonic growth and development, and a successful pregnancy depends on the dynamic balance of the microenvironment at the maternal-fetal interface.
Qian Chen +16 more
doaj +1 more source
Intracerebral Hemorrhage and Ischemic Stroke of Different Etiologies Have Distinct Alternatively Spliced mRNA Profiles in the Blood: a Pilot RNA-seq Study. [PDF]
, 2015 Whole transcriptome studies have used 3'-biased expression microarrays to study genes regulated in the blood of stroke patients. However, alternatively spliced messenger RNA isoforms have not been investigated for ischemic stroke or intracerebral ...
Ander, Bradley P +8 more
core +2 more sources
Molecular recording of mammalian embryogenesis. [PDF]
, 2019 Ontogeny describes the emergence of complex multicellular organisms from single totipotent cells. This field is particularly challenging in mammals, owing to the indeterminate relationship between self-renewal and differentiation, variation in progenitor
Adamson, Britt +13 more
core +2 more sources
Linked optical and gene expression profiling of single cells at high-throughput. [PDF]
, 2020 Single-cell RNA sequencing has emerged as a powerful tool for characterizing cells, but not all phenotypes of interest can be observed through changes in gene expression.
Abate, Adam R +5 more
core +1 more source
Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
, 2018 All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
core +2 more sources
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines [PDF]
, 2017 Neuroblastoma cell lines are an important and cost-effective model used to study oncogenic drivers of the disease. While many of these cell lines have been previously characterized with SNP, methylation, and/or mRNA expression microarrays, there has not ...
Adams, Rebecca N. +9 more
core +5 more sources
Technology in Cancer Research & Treatment, 2022 Introduction: The application of single-cell RNA sequencing to delineate tissue heterogeneity and complexity has become increasingly popular. Given its tremendous resolution and high-dimensional capacity for in-depth investigation, single-cell RNA ...
GS Chuwdhury MPhil +2 more
doaj +1 more source
Simultaneous profiling of transcriptome and DNA methylome from a single cell. [PDF]
, 2016 BackgroundSingle-cell transcriptome and single-cell methylome technologies have become powerful tools to study RNA and DNA methylation profiles of single cells at a genome-wide scale. A major challenge has been to understand the direct correlation of DNA
An, Qin +9 more
core +3 more sources