Delineating COVID-19 immunological features using single-cell RNA sequencing
The Innovation, 2022 Understanding the molecular mechanisms of coronavirus disease 2019 (COVID-19) pathogenesis and immune response is vital for developing therapies. Single-cell RNA sequencing has been applied to delineate the cellular heterogeneity of the host response ...
Wendao Liu +6 more
doaj +1 more source
Improved annotation of 3' untranslated regions and complex loci by combination of strand-specific direct RNA sequencing, RNA-seq and ESTs [PDF]
, 2014 The reference annotations made for a genome sequence provide the framework for all subsequent analyses of the genome. Correct and complete annotation in addition to the underlying genomic sequence is particularly important when interpreting the results ...
Junfang Song +29 more
core +1 more source
Single Cell RNA Sequencing of Rare Immune Cell Populations
Frontiers in Immunology, 2018 Single-cell RNA sequencing (scRNA-Seq) is transforming our ability to characterize cells, particularly rare cells that are often overlooked in bulk population analytical approaches.
Akira Nguyen +9 more
doaj +1 more source
Microglia Heterogeneity in Alzheimer’s Disease: Insights From Single-Cell Technologies
Frontiers in Synaptic Neuroscience, 2021 Microglia are resident immune cells in the central nervous system and play critical roles in brain immunity, development, and homeostasis. The pathology of Alzheimer’s disease (AD) triggers activation of microglia.
Hansen Wang
doaj +1 more source
An Innovative Approach to Tissue Processing and Cell Sorting of Fixed Cells for Subsequent Single-Cell RNA Sequencing [PDF]
, 2022 Although single-cell RNA sequencing (scRNA-seq) is currently the gold standard for the analysis of cell-specific expression profiles, the options for processing, staining, and preserving fresh cells remain very limited.
Lukas Bäriswyl +7 more
core +1 more source
Pumping the brakes on RNA velocity by understanding and interpreting RNA velocity estimates
Genome Biology, 2023 Background RNA velocity analysis of single cells offers the potential to predict temporal dynamics from gene expression. In many systems, RNA velocity has been observed to produce a vector field that qualitatively reflects known features of the system ...
Shijie C. Zheng +4 more
doaj +1 more source
Single cell RNA sequencing research in maternal fetal interface
Frontiers in Cell and Developmental Biology, 2023 The maternal-fetal interface is an essential environment for embryonic growth and development, and a successful pregnancy depends on the dynamic balance of the microenvironment at the maternal-fetal interface.
Qian Chen +16 more
doaj +1 more source
Development of methods for the analysis of deep sequencing data; applications to the discovery of functions of RNA-binding proteins [PDF]
, 2014 With the recent advances in nucleotide sequencing technologies, it became easy to generate tens of millions of reads with genome- or transcriptome-wide distribution with reduced cost and high accuracy.
Bilen, Biter
core +1 more source
Single-cell RNA sequencing of human kidney
, 2023 A comprehensive cellular anatomy of normal human kidney is crucial to address the cellular origins of renal disease and renal cancer. Some kidney diseases may be cell type-specific, especially renal tubular cells.
huan yang (17600760)
core +1 more source
Technology in Cancer Research & Treatment, 2022 Introduction: The application of single-cell RNA sequencing to delineate tissue heterogeneity and complexity has become increasingly popular. Given its tremendous resolution and high-dimensional capacity for in-depth investigation, single-cell RNA ...
GS Chuwdhury MPhil +2 more
doaj +1 more source