Results 271 to 280 of about 3,551,300 (318)
Some of the next articles are maybe not open access.
Trends in Biochemical Sciences, 2021
The inability to make broad, minimally biased measurements of a cell's proteome stands as a major bottleneck for understanding how gene expression translates into cellular phenotype. Unlike sequencing for nucleic acids, there is no dominant method for making single-cell proteomic measurements.
Luke F, Vistain, Savaş, Tay
openaire +2 more sources
The inability to make broad, minimally biased measurements of a cell's proteome stands as a major bottleneck for understanding how gene expression translates into cellular phenotype. Unlike sequencing for nucleic acids, there is no dominant method for making single-cell proteomic measurements.
Luke F, Vistain, Savaş, Tay
openaire +2 more sources
Current Opinion in Biotechnology, 2003
Electroporation is a widely used method for the introduction of polar and charged agents such as dyes, drugs, DNA, RNA, proteins, peptides, and amino acids into cells. Traditionally, electroporation is performed with large electrodes in a batch mode for treatment of a large number of cells in suspension.
Jessica, Olofsson +5 more
openaire +2 more sources
Electroporation is a widely used method for the introduction of polar and charged agents such as dyes, drugs, DNA, RNA, proteins, peptides, and amino acids into cells. Traditionally, electroporation is performed with large electrodes in a batch mode for treatment of a large number of cells in suspension.
Jessica, Olofsson +5 more
openaire +2 more sources
Nature Methods, 2011
Methods for genomic analysis at single-cell resolution enable new understanding of complex biological phenomena. Single-cell techniques, ranging from flow cytometry and microfluidics to PCR and sequencing, are used to understand the cellular composition of complex tissues, find new microbial species and perform genome-wide haplotyping.
Tomer, Kalisky, Stephen R, Quake
openaire +2 more sources
Methods for genomic analysis at single-cell resolution enable new understanding of complex biological phenomena. Single-cell techniques, ranging from flow cytometry and microfluidics to PCR and sequencing, are used to understand the cellular composition of complex tissues, find new microbial species and perform genome-wide haplotyping.
Tomer, Kalisky, Stephen R, Quake
openaire +2 more sources
Pflügers Archiv - European Journal of Physiology, 2001
Using modified patch-clamp methodology, we demonstrated that it is possible to insert genes or other compounds routinely into single cells by electroporation. When the cell is indented by a small-tipped microelectrode, a voltage of 10 V or less in the pipette is divided by the pipette resistance and the series resistance of the cleft between the ...
James L, Rae, Richard A, Levis
openaire +2 more sources
Using modified patch-clamp methodology, we demonstrated that it is possible to insert genes or other compounds routinely into single cells by electroporation. When the cell is indented by a small-tipped microelectrode, a voltage of 10 V or less in the pipette is divided by the pipette resistance and the series resistance of the cleft between the ...
James L, Rae, Richard A, Levis
openaire +2 more sources
Science, 1983
Both photosynthetic and nonphotosynthetic microorganisms, grown on various carbon and energy sources, are used in fermentation processes for the production of single-cell proteins. Commercial-scale production has been limited to two algal processes, one bacterial process, and several yeast and fungal processes.
openaire +2 more sources
Both photosynthetic and nonphotosynthetic microorganisms, grown on various carbon and energy sources, are used in fermentation processes for the production of single-cell proteins. Commercial-scale production has been limited to two algal processes, one bacterial process, and several yeast and fungal processes.
openaire +2 more sources
New England Journal of Medicine, 2006
Dr. Susan Okie discusses the controversy surrounding a recent report suggesting that new stem-cell lines may be generated while preserving embryos. On the contrary, all donated embryos used in the study were destroyed during the experiments.
openaire +2 more sources
Dr. Susan Okie discusses the controversy surrounding a recent report suggesting that new stem-cell lines may be generated while preserving embryos. On the contrary, all donated embryos used in the study were destroyed during the experiments.
openaire +2 more sources
Current Opinion in Plant Biology, 2005
The evolution of higher plants depended on the ability of cells to express hereditary information in many different ways and led to the development of specialized cell types, reflecting an elaborate system of control over gene expression in the individual component cells of various tissues. Bulk tissue sampling results in the loss of spatial resolution,
openaire +2 more sources
The evolution of higher plants depended on the ability of cells to express hereditary information in many different ways and led to the development of specialized cell types, reflecting an elaborate system of control over gene expression in the individual component cells of various tissues. Bulk tissue sampling results in the loss of spatial resolution,
openaire +2 more sources