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Microfluidics for single cell analysis
Current Opinion in Biotechnology, 2012Substantial evidence shows that the heterogeneity of individual cells within a genetically identical population can be critical to their chance of survival. Methods that use average responses from a population often mask the difference from individual cells.
Huabing, Yin, Damian, Marshall
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Chemical Analysis of Single Cells
Annual Review of Analytical Chemistry, 2008Chemical analysis of single cells requires methods for quickly and quantitatively detecting a diverse array of analytes from extremely small volumes (femtoliters to nanoliters) with very high sensitivity and selectivity. Microelectrophoretic separations, using both traditional capillary electrophoresis and emerging microfluidic methods, are well ...
Laura M, Borland +3 more
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Single cell analysis at the nanoscale
Chem. Soc. Rev., 2012The fundamental life processes such as signal transduction, intracellular trafficking, protein degradation, and DNA repair often occur in nanometric subcellular compartments. It is essential to conduct single cell analysis specifically at the nanoscale to fully understand the critical cellular processes while providing important medical applications ...
Zheng, Xin Ting, Li, Chang Ming
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Single-Cell RNAseq Analysis of lncRNAs
2021Mammalian genomes are pervasively transcribed and a small fraction of RNAs produced codify for proteins. The importance of noncoding RNAs for the maintenance of cell functions is well known (e.g., rRNAs, tRNAs), but only recently it was first demonstrated the involvement of microRNAs (miRNAs) in posttranscriptional regulation and then the activity of ...
Cagnin S. +3 more
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Single Cell Analysis of Proteoforms
Journal of Proteome ResearchWe introduce single cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enable a scalable approach to single cell proteomics.
Pei Su +12 more
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Analytical and Bioanalytical Chemistry, 2006
Why is it important to study single cells? Scientists have long pondered the purpose of increasingly smaller biological entities within various organisms, with the cell acknowledged to be one of the fundamental building blocks of life. Furthermore, individual cells have discrete molecular, metabolic, and proteomic identities. Analogous to the pieces of
Jonathan V. Sweedler, Edgar A. Arriaga
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Why is it important to study single cells? Scientists have long pondered the purpose of increasingly smaller biological entities within various organisms, with the cell acknowledged to be one of the fundamental building blocks of life. Furthermore, individual cells have discrete molecular, metabolic, and proteomic identities. Analogous to the pieces of
Jonathan V. Sweedler, Edgar A. Arriaga
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Single-cell analysis of unstable genes
Journal of Assisted Reproduction and Genetics, 1996We have developed sensitive diagnostic procedures for studies on the normal and mutant alleles of the triplet repeat genes associated with myotonic dystrophy and fragile X in single human somatic cells, gametes and embryos.Polymerase chain reaction (PCR) assays for the normal alleles of the myotonic dystrophy and fragile X loci have been refined to the
R, Daniels +3 more
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Microfluidic Device for Single-Cell Analysis
Analytical Chemistry, 2003We have developed a novel microfluidic device constructed from poly(dimethylsiloxane) using multilayer soft lithography technology for the analysis of single cells. The microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension, and integrated valves and pumps enable the precise delivery of nanoliter ...
Aaron R, Wheeler +8 more
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The Single-Cell Lab or How to Perform Single-Cell Molecular Analysis
2015The increasing interest towards cellular heterogeneity within cell populations has pushed the development of new protocols to isolate and analyze single cells. PCR-based amplification techniques are widely used in this field. However, setting up an experiment and analyzing the results can sometimes be challenging.
Roland, Kirchner +1 more
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