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Chemical Analysis of Single Cells [PDF]
Annual Review of Analytical Chemistry, 2008 Chemical analysis of single cells requires methods for quickly and quantitatively detecting a diverse array of analytes from extremely small volumes (femtoliters to nanoliters) with very high sensitivity and selectivity. Microelectrophoretic separations, using both traditional capillary electrophoresis and emerging microfluidic methods, are well ...
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Analytical and Bioanalytical Chemistry, 2006
Why is it important to study single cells? Scientists have long pondered the purpose of increasingly smaller biological entities within various organisms, with the cell acknowledged to be one of the fundamental building blocks of life. Furthermore, individual cells have discrete molecular, metabolic, and proteomic identities. Analogous to the pieces of
Edgar A. Arriaga, Jonathan V. Sweedler
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Why is it important to study single cells? Scientists have long pondered the purpose of increasingly smaller biological entities within various organisms, with the cell acknowledged to be one of the fundamental building blocks of life. Furthermore, individual cells have discrete molecular, metabolic, and proteomic identities. Analogous to the pieces of
Edgar A. Arriaga, Jonathan V. Sweedler
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Single-Cell Transcriptional Analysis
Annual Review of Analytical Chemistry, 2017Despite being a relatively recent technological development, single-cell transcriptional analysis through high-throughput sequencing has already been used in hundreds of fruitful studies to make exciting new biological discoveries that would otherwise be challenging or even impossible.
Wu, Angela Ruohao +3 more
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Microfluidics for single cell analysis
2022Cells have several internal molecules that are present in low amounts and any fluctuation in its number drives a change in cell behavior. These molecules present inside the cells are continuously fluctuating, thus producing noises in the intrinsic environment and thereby directly affecting the cellular behavior. Single-cell analysis using microfluidics
Rupesh, Maurya +5 more
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Integrative single-cell analysis
Nature Reviews Genetics, 2019The recent maturation of single-cell RNA sequencing (scRNA-seq) technologies has coincided with transformative new methods to profile genetic, epigenetic, spatial, proteomic and lineage information in individual cells. This provides unique opportunities, alongside computational challenges, for integrative methods that can jointly learn across multiple ...
Tim Stuart, Rahul Satija
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Microfluidics for single cell analysis
Current Opinion in Biotechnology, 2012Substantial evidence shows that the heterogeneity of individual cells within a genetically identical population can be critical to their chance of survival. Methods that use average responses from a population often mask the difference from individual cells.
Huabing Yin, Damian Marshall
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Single-cell analysis at the threshold
Nature Biotechnology, 2016A discussion of some of the challenges and promise of single-cell technology. ; © 2016 Nature Publishing Group. Published 08 November 2016.
Chen, Xi +7 more
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Single-Cell RNAseq Analysis of lncRNAs
2021Mammalian genomes are pervasively transcribed and a small fraction of RNAs produced codify for proteins. The importance of noncoding RNAs for the maintenance of cell functions is well known (e.g., rRNAs, tRNAs), but only recently it was first demonstrated the involvement of microRNAs (miRNAs) in posttranscriptional regulation and then the activity of ...
Cagnin S. +3 more
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