Results 1 to 10 of about 314,376 (117)

Current best practices in single‐cell RNA‐seq analysis: a tutorial

Molecular Systems Biology, 2019
Single‐cell RNA‐seq has enabled gene expression to be studied at an unprecedented resolution. The promise of this technology is attracting a growing user base for single‐cell analysis methods. As more analysis tools are becoming available, it is becoming
Malte D Luecken, Fabian J Theis
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Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity

Cell Reports, 2017
The hypothalamus is one of the most complex brain structures involved in homeostatic regulation. Defining cell composition and identifying cell-type-specific transcriptional features of the hypothalamus is essential for understanding its functions and ...
Renchao Chen, Xiaoji Wu, Lan Jiang, Yi Zhang   +3 more
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Methods, Challenges and Potentials of Single Cell RNA-seq

Biology, 2012
RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene
Daniel Hebenstreit
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A map of bat virus receptors derived from single-cell multiomics

Scientific Data, 2022
Measurement(s) RNA-seq gene expression profiling assay • ATAC-Seq Technology Type(s) RNA-seq of coding RNA from single cells • Single cell ATAC-seq (cell index) Sample Characteristic - Organism Rhinolophus ...
Tianhang Lv, Xiaoshan Wang, Chao Yu, Zhifeng Wang, Rong Xiang, Linmiao Li, Yue Yuan, Yuhang Wang, Xiaoyu Wei, Yeya Yu, Xiangyang He, Libiao Zhang, Qiuting Deng, Peiying Wu, Yong Hou, Jinping Chen, Chuanyu Liu, Gary Wong, Longqi Liu   +18 more
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Single‐cell RNA‐seq and bulk RNA‐seq explore the prognostic value of exhausted T cells in hepatocellular carcinoma

IET Systems Biology, 2023
Hepatocellular carcinoma (HCC) remains a worldwide health problem. Mounting evidence indicates that exhausted T cells play a critical role in the progress and treatment of HCC.
Xiaolong Tang, Yandong Miao, Lixia Yang, Wuhua Ha, Zheng Li, Denghai Mi   +5 more
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LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis

Genome Biology, 2023
Existing single-cell RNA sequencing (scRNA-seq) methods rely on reverse transcription (RT) and second-strand synthesis (SSS) to convert single-stranded RNA into double-stranded DNA prior to amplification, with the limited RT/SSS efficiency compromising ...
Jun Lyu, Chongyi Chen
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Characterizing the composition of iPSC derived cells from bulk transcriptomics data with CellMap

Scientific Reports, 2022
Induced pluripotent stem cell (iPSC) derived cell types are increasingly employed as in vitro model systems for drug discovery. For these studies to be meaningful, it is important to understand the reproducibility of the iPSC-derived cultures and their ...
Zhengyu Ouyang, Nathanael Bourgeois-Tchir, Eugenia Lyashenko, Paige E. Cundiff, Patrick F. Cullen, Ravi Challa, Kejie Li, Xinmin Zhang, Fergal Casey, Sandra J. Engle, Baohong Zhang, Maria I. Zavodszky   +11 more
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Projecting RNA measurements onto single cell atlases to extract cell type-specific expression profiles using scProjection

Nature Communications, 2023
Multi-modal single cell RNA assays capture RNA content as well as other data modalities, such as spatial cell position or the electrophysiological properties of cells.
Nelson Johansen, Hongru Hu, Gerald Quon
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SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology

Genome Biology, 2018
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq).
Mahmoud N. Abdelmoez, Kei Iida, Yusuke Oguchi, Hidekazu Nishikii, Ryuji Yokokawa, Hidetoshi Kotera, Sotaro Uemura, Juan G. Santiago, Hirofumi Shintaku   +8 more
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Integrated analysis of glycan and RNA in single cells

iScience, 2021
Summary: Single-cell sequencing has emerged as an indispensable technology to dissect cellular heterogeneity but never been applied to the simultaneous analysis of glycan and RNA.
Fumi Minoshima, Haruka Ozaki, Haruki Odaka, Hiroaki Tateno   +3 more
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