Results 121 to 130 of about 731,457 (305)

Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencing

, 2020
The libraries generated by high-throughput single cell RNA-sequencing (scRNA-seq) platforms such as the Chromium from 10x Genomics require considerable amounts of sequencing, typically due to the large number of cells.
Senabouth, A (15675581), Pebay, A (15628247), Shi, Q (15755174), Beckhouse, A (15755180), Kristof Wing (14753113), Fink, L (15755177), Daniszewski, M (15633953), Shi, L (15615614), Hung, SSC (15629483), Alexander Hewitt (14739028), Jiang, F (15622742), Andersen, S (15709130), Lukowski, SW (15675587), Powell, JE (15633947), Zhang, W (15524786)   +14 more
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Single-Cell RNA Sequencing Reveals Cellular Heterogeneity in an Acral Amelanotic Melanoma After Immunotherapy Treatment

, 2023
Le Zhuang,1– 6,* Jie Tian,1– 4,7,* Binbin Lai,1– 4,7 Guohong Zhang,1– 4 Hang Li1– 4 1Department of Dermatology, Peking University First Hospital, Beijing, People’s Republic of China; 2National Clinical Research Center for Skin and Immune Diseases,
Tian J, Zhang G, Li H, Lai B, Zhuang L
core  

Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species [PDF]

, 2012
Conclusion: By carefully choosing the appropriate RNA extraction method, whole blood can become a valuable source for high-throughput applications like expression arrays or transcriptome sequencing from natural populations.
Serieys, Laurel EK, Thalmann, Olaf, Thalmann Olaf, Schwochow Doreen, Serieys Laurel EK, Wayne, Robert K., Robert K Wayne, Doreen Schwochow, Laurel EK Serieys, Serieys, Laurel E.K., Schwochow, Doreen, Wayne Robert K, Olaf Thalmann, Wayne, Robert K   +13 more
core   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

lute: estimating the cell composition of heterogeneous tissue with varying cell sizes using gene expression

BMC Genomics
Background Relative cell type fraction estimates in bulk RNA-sequencing data are important to control for cell composition differences across heterogenous tissue samples.
Sean K. Maden, Louise A. Huuki-Myers, Sang Ho Kwon, Leonardo Collado-Torres, Kristen R. Maynard, Stephanie C. Hicks   +5 more
doaj   +1 more source

A functional genomic analysis of cell morphology using RNA interference [PDF]

, 2003
Background: The diversity of metazoan cell shapes is influenced by the dynamic cytoskeletal network. With the advent of RNA-interference (RNAi) technology, it is now possible to screen systematically for genes controlling specific cell-biological ...
Coulson, A., Echeverri C, Coulson, A, Perrimon N, Kiger AA, Coulson A, Jones, MR, Perrimon, Norbert, Jones, S, Echeverri, C., Jones MR, Baum, B., Jones, M.R., Baum, B, Baum B, Kiger, AA, Perrimon, N., Echeverri, C, Jones, S., Jones S, Kiger, A.A.   +20 more
core   +1 more source

Hippo pathway at the crossroads of stemness and therapeutic resistance in breast cancer

Molecular Oncology, EarlyView.
Dysregulation of the Hippo pathway drives nuclear accumulation of YAP/TAZ, activating stemness‐related transcriptional programs that sustain breast cancer stemness and fuel therapeutic resistance across subtypes, underscoring Hippo signaling as a targetable vulnerability. Figure created and edited with BioRender.com.
Giulia Schiavoni, Antonella Palmese, Stefano Scalera, Laura Cipriani, Davide Mascolo, Patrizia Vici, Teresa Arcuri, Lorena Filomeno, Eriseld Krasniqi, Giovanni Blandino, Giulia Bon, Marcello Maugeri‐Saccà   +11 more
wiley   +1 more source

Single-nuclei transcriptome analysis of IgM+ cells isolated from channel catfish (Ictalurus punctatus) spleen

Frontiers in Immunology
Catfish production is the primary aquaculture sector in the United States, and the key cultured species is channel catfish (Ictalurus punctatus). The major causes of production losses are pathogenic diseases, and the spleen, an important site of adaptive
Johanna E. Aldersey, Johanna E. Aldersey, Jason W. Abernathy, Benjamin H. Beck, Miles D. Lange   +4 more
doaj   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

scGSECA:identification of altered biological processes in single-cell RNA-sequencing data by discretization of expression profiles

, 2023
Bimodality of the distribution of expression levels has been observed in bulk RNA-seq. Two possible explanations underpin this kind of partitioning and refer to opposite paradigmatic models.
Salvatore Oliviero, Michele Caselle, Matteo Cereda, Andrea Lauria, Serena Peirone, Francesca Priante, Sarah Perrone   +6 more
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