Results 81 to 90 of about 731,457 (305)
Stably expressed genes in single-cell RNA-sequencing [PDF]
Journal of Bioinformatics and Computational Biology, 2018 Abstract Motivation In single-cell RNA-sequencing (scRNA-seq) experiments, RNA transcripts are extracted and measured from isolated cells to understand gene expression at the cellular level. Measurements from this technology are affected by many technical artifacts, including batch effects.
Deeke, Julie M. +1 more
openaire +2 more sources
Single-cell RNA sequencing v1 [PDF]
, 2021 Single-cell RNA sequencing (scRNA-seq) allows for transcriptional profiling of individual cells within a heterogenous sample. This protocol describes a method for performing scRNA-seq using handpicked pancreatic islets from organ donors.
Klaus H. Kaestner Lab, Suzanne Shapira
openaire +1 more source
Single-cell long-read targeted sequencing reveals transcriptional variation in ovarian cancer
Nature CommunicationsSingle-cell RNA sequencing predominantly employs short-read sequencing to characterize cell types, states and dynamics; however, it is inadequate for comprehensive characterization of RNA isoforms. Long-read sequencing technologies enable single-cell RNA
Ashley Byrne +12 more
doaj +1 more source
, 2023 Dan He,1,* Qiao Yu,2,* Xiaona Zeng,2,* Jihua Feng,2 Ruiqi Yang,2 Huan Wan,2 Ying Zhong,2 Yanli Yang,2 Ruzhi Zhao,2 Junyu Lu,3,4 Jianfeng Zhang1,2,4 1Department of General Practice, The Second Affiliated Hospital of Guangxi Medical University,
He D +10 more
core
The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes
FEBS Letters, EarlyView.Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga +3 more
wiley +1 more source
Power Analysis of Single Cell RNA-Sequencing Experiments [PDF]
Nature Methods, 2016 Abstract High-throughput single cell RNA sequencing (scRNA-seq) has become an established and powerful method to investigate transcriptomic cell-to-cell variation, and has revealed new cell types, and new insights into developmental process and stochasticity in gene expression.
Svensson, Valentine +7 more
openaire +5 more sources
FEBS Letters, EarlyView.Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil +4 more
wiley +1 more source
Diversity of Rainbow Trout Blood B Cells Revealed by Single Cell RNA Sequencing
, 2021 Single-cell sequencing technologies capable of providing us with immune information from dozens to thousands of individual cells simultaneously have revolutionized the field of immunology these past years.
Pedro Perdiguero +6 more
core +1 more source
FEBS Letters, EarlyView.Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai +4 more
wiley +1 more source
Single-cell RNA sequencing of human kidney
, 2019 A comprehensive cellular anatomy of normal human kidney is crucial to address the cellular origins of renal disease and renal cancer. Some kidney diseases may be cell type-specific, especially renal tubular cells.
Zhenyuan Yu (6698111) +2 more
core +1 more source