Results 121 to 130 of about 5,030,666 (315)

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Single-Molecule Imaging of Bmp4 Dimerization on Human Periodontal Ligament Cells

, 2012
We expressed bone morphogenetic protein 4 (BMP4) fused with enhanced green fluorescent protein (BMP4-EGFP) in the secretory pathways of producer cells. Fluorescent EGFP was acquired only after we interrupted the transport of BMP4- EGFP by culturing cells
秘心吾;姜昱至;周綠蘋;林俊彬, MI, HSIN-WU;CHIANG, YU-CHIH;CHOW, LU-PING;LIN, CHUN-PIN   +1 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids [PDF]

, 2007
The bacteriophage phi29 generates large forces to compact its double-stranded DNA genome into a protein capsid by means of a portal motor complex. Several mechanical models for the generation of these high forces by the motor complex predict coupling of ...
Hetherington, Craig L., Craig L Hetherington, Falk, Wayne, Wayne Falk, Thorsten Hugel, Jardine, Paul J., Falk Wayne, Hugel Thorsten, Bustamante, Carlos, Grimes Shelley, Michaelis, Jens, Walter Jessica M, Bustamante Carlos, Michaelis Jens, Anderson, Dwight L., Hugel, Thorsten, Grimes, Shelley, Paul J Jardine, Jessica M Walter, Walter, Jessica M., Jardine Paul J, Dwight L Anderson, Jens Michaelis, Anderson Dwight L, Carlos Bustamante, Hetherington Craig L, Shelley Grimes   +26 more
core   +1 more source

Pharmacologic hyperstabilisation of the HIV-1 capsid lattice induces capsid failure

eLife
The HIV-1 capsid has emerged as a tractable target for antiretroviral therapy. Lenacapavir, developed by Gilead Sciences, is the first capsid-targeting drug approved for medical use.
KM Rifat Faysal, James C Walsh, Nadine Renner, Chantal L Márquez, Vaibhav B Shah, Andrew J Tuckwell, Michelle P Christie, Michael W Parker, Stuart G Turville, Greg J Towers, Leo C James, David A Jacques, Till Böcking   +12 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

High-throughput multispot single-molecule spectroscopy

, 2010
Solution-based single-molecule spectroscopy and fluorescence correlation spectroscopy (FCS) are powerful techniques to access a variety of molecular properties such as size, brightness, conformation, and binding constants. However, this is limited to low
MARANGONI, STEFANO, S. Weiss, RECH, IVAN, Xavier Michalet, T. Kim, Sergio Cova, Taiho Kim, GHIONI, MASSIMO ANTONIO, Massimo Ghioni, Ryan A. Colyer, Stefano Marangoni, COVA, SERGIO, G. Scalia, Ivan Rech, X. Michalet, Daniele Resnati, RESNATI, DANIELE, R. A. Colyer, Shimon Weiss, Giuseppe Scalia   +19 more
core   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Single Molecule Fluorescence Imaging of Nanoconfinement in Porous Materials

Single-molecule fluorescence (SMF) microscopy imaging was employed to understand the nanoconfinement in porous materials. The changes in chemical and physical properties of molecules in nanoconfined space are often termed as the confinement effects.
Mansour, Nourhan
core   +1 more source