Results 151 to 160 of about 5,030,666 (315)

Single stranded DNA: A new model system for single molecule polymer dynamics

open access: yes, 2011
The study of polymer dynamics has been an active area of research for many years. For nearly two decades, fluorescently-labeled double stranded DNA (dsDNA) has been the model system for studying single molecule polymer dynamics in non-equilibrium ...
Brockman, Christopher A.
core  

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

open access: yesFEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Toward Practical Single‐Molecule/Atom Switches

open access: yesAdvanced Science
Electronic switches have been considered to be one of the most important components of contemporary electronic circuits for processing and storing digital information.
Xiaona Xu   +4 more
doaj   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

open access: yesFEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson   +6 more
wiley   +1 more source

Catching the active form of a protein by single molecule methodologies

open access: yes, 2007
The structural disorder of intrinsically unstructured proteins is the outcome of a complex ensemble of conformers driven by a rugged energy landscape.
SAMORI', BRUNO
core  

Molecular dimer junctions forming: Role of disulfide bonds and electrode‐compression‐time

open access: yesSmartMat
Thanks to their excellent bond strength, phenyl‐based molecules with thiol anchoring groups are extensively employed to form stable single‐molecule junctions.
Xueyan Zhao   +11 more
doaj   +1 more source

Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes

open access: yesFEBS Letters, EarlyView.
Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez   +2 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Single-molecule study of the DNA helicase regulating genomic stability

open access: yes, 2011
A DNA helicase, PcrA is an essential protein in gram-positive bacteria. PcrA utilizes its helicase activity and translocation activity to regulate various cellular functions including plasmid replication and counteracting deleterious recombination ...
Park, Jeehae
core  

In Situ Reconnection of Nanoelectrodes Over 20 nm Gaps on Polyimide Substrate

open access: yesSmall Structures
The current densities in nowadays electronic circuitry are close to the electromigration threshold that may result in the fracture of circuits due to electromigration, hampering further miniaturization of integrated chips.
Xubin Zhang   +11 more
doaj   +1 more source

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