Results 181 to 190 of about 1,093 (205)
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Multiplexed Single‐Nucleus RNA Sequencing Using Lipid‐Oligo Barcodes
Current Protocols, 2022AbstractThis protocol describes a robust pipeline for simultaneously analyzing multiple samples by single‐nucleus (sn)RNA‐seq. cDNA obtained from each single sample are labeled with the same lipid‐coupled oligonucleotide barcode (10X Genomics). Nuclei from as many as 12 individual samples can be pooled together and simultaneously processed for cDNA ...
Qi, Zhang +6 more
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Single nucleus RNA-sequencing: how it's done, applications and limitations
Emerging Topics in Life Sciences, 2021Single nuclei RNA-sequencing (sNuc-Seq) is a methodology which uses isolated nuclei instead of whole cells to profile gene expression. By using droplet microfluidic technologies, users are able to profile thousands of single transcriptomes at high throughput from their chosen tissue.
Juliane Fischer, Thomas Ayers
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Journal of Dermatological Science, 2023
Single-cell RNA sequencing (scRNA-seq) has advanced our understanding of skin biology, but its utility is restricted by the requirement of fresh samples, inadequate dissociation-induced cell loss or death, and activation during tissue digestion. Single-nucleus RNA sequencing (snRNA-seq) can use frozen, hard-to-dissociate materials, which might be a ...
Ronghui Zhu +2 more
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Single-cell RNA sequencing (scRNA-seq) has advanced our understanding of skin biology, but its utility is restricted by the requirement of fresh samples, inadequate dissociation-induced cell loss or death, and activation during tissue digestion. Single-nucleus RNA sequencing (snRNA-seq) can use frozen, hard-to-dissociate materials, which might be a ...
Ronghui Zhu +2 more
exaly +3 more sources
Large-Scale Single-Nucleus RNA Sequencing Compatible with Complex Archived Samples
2022Transcriptome profiling at single-cell resolution allows us to identify and assess functional cell types and cellular states, including those within degenerating ocular tissues in retinitis pigmentosa. The technology is particularly valuable when studying tissues with high cellular heterogeneity, or when specific cell types are of interest.
Chao-Yu, Liu, Hsu-Hsin, Chen
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Single-nucleus RNA sequencing of diabetic mice
Mice were deeply anesthetized with isoflurane and hippocampus tissues were rapidly dissected for snRNA-seq.openaire +1 more source
Single-nucleus RNA sequencing of rostral ventromedial medulla in mice with trigeminal neuralgia
International Journal of NeuroscienceAim: To investigate the transcriptional changes and cell interactions following trigeminal neuralgia (TN) in different cell types in rostral ventromedial medulla (RVM).Methods: In this study, trigeminal neuropathic pain was induced in mice by ligating the left infraorbital nerve. Ten days after nerve ligation, we performed single-nucleus RNA sequencing
Xia, Zhang +6 more
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Single-nucleus RNA sequencing of Macaque hippocampus
Single-nucleus RNA sequencing of Macaque ...openaire +1 more source
Single Nucleus Total RNA Sequencing of Formalin‐Fixed Paraffin‐Embedded Gliomas
Small MethodsAbstractGliomas, the predominant form of brain cancer, comprise diverse malignant subtypes with limited curative therapies available. The insufficient understanding of their molecular diversity and evolutionary processes hinders the advancement of new treatments.
Ziye Xu +12 more
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