Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Designing for Green and Grey: Insights from Single-Use Plastic Water Bottles. [PDF]
Int J Environ Res Public Health, 2022 Kim T, Lee SD.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Towards a sustainable campus: zero single-use plastic campaign
, 2020 Plastics are man made polymeric organic materials, which can be moulded into many shapes for various uses. They are lightweight, durable and long-lasting and usable for multiple purposes.
Zainan Nazri, Nurul Jannah +2 more
core
Plastic Perception: An Analysis on Single-Use Consumerism [PDF]
, 2018 Plastic has become essential to everyday use as the material is very costeffective and therefore used to create various single-use products. According to the Ocean Conservancy, the amount of ocean plastic in weight will outweigh fish by 2050.
Beck, Lindsay +2 more
core
Opportunities for single-use plastic reduction in the food service sector during COVID-19. [PDF]
Sustain Prod Consum, 2022 Molloy S, Varkey P, Walker TR.
europepmc +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
FEBS Open Bio, EarlyView.GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Drivers of public plastic (mis)use - New insights from changes in single-use plastic usage during the Covid-19 pandemic. [PDF]
Sci Total Environ, 2022 Winton D, Marazzi L, Loiselle S.
europepmc +1 more source
FEBS Open Bio, EarlyView.Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source