Results 121 to 130 of about 12,921,630 (316)
Hierarchical mechanism of amino acid sensing by the T-box riboswitch
Nature Communications, 2018 Riboswitches on 5′ ends of mRNAs are important for bacterial gene regulation. Here the authors probe the mechanism of a tRNA aminoacylation sensing T-box riboswitch using single-molecule fluorescence microscopy to characterize dynamic solution ...
Krishna C. Suddala +5 more
doaj +1 more source
Diversity and complexity in neural organoids
FEBS Letters, EarlyView.Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley +1 more source
Population-level transcription cycles derive from stochastic timing of single-cell transcription
, 2009 Eukaryotic transcription is a dynamic process relying on a large number of proteins. By measuring the cycling expression of the pyruvate dehydrogenase kinase 4 gene in human cells, we constructed a detailed stochastic model for single-gene transcription ...
Carlberg, C. +13 more
core +1 more source
Plant Single Cell Type Systems Biology [PDF]
, 2016 The phenotype of a plant in response to a stress condition is the reflection of the molecular responses in different cell-types composing the plant. The multicellular complexity represents a challenge when accessing specific responses of each cell or ...
Libault, Marc +3 more
core +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
DEVELOPMENT AND APPLICATION OF SINGLE CELL MASS SPECTROMETRY TECHNIQUES FOR NON-ADHERENT CELL ANALYSIS [PDF]
, 2020 Mass spectrometry (MS) analysis of biological samples is traditionally carried out using extractions from large populations of cells, concealing the information from individual cells.
Zhu, Yanlin
core
FEBS Letters, EarlyView.Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley +1 more source
Single-cell genotyping workflow implementing the Single-Cell Printer.
, 2016 (A) The cell suspension is filled into the sterile single-use cartridge. (B) The microwell plate holder is equipped with a camera to automatically determine and adjust for the dispenser offset prior to cell printing (automatic offset compensation, AOC ...
Björn Hackanson (14623) +14 more
core +1 more source