Results 131 to 140 of about 3,900,612 (318)
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Heat Shock Factor HSFA6b Mediates Mitochondrial Unfolded Protein Response in Arabidopsis thaliana
PlantsMitochondria are important organelles in eukaryotes and are involved in various metabolic processes. Mitochondrial proteotoxic stress triggers the mitochondrial unfolded protein response (UPRmt) to restore mitochondrial protein homeostasis and maintain ...
Guolong Yu +6 more
doaj +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
The hidden mycobiome: emerging role in anticancer immunotherapy
MycologyAs one of the emerging hallmarks, the mycobiome has recently gained significant recognition in cancer research, especially regarding anticancer immunotherapy.
Ze-Chang Peng +6 more
doaj +1 more source
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
MICROBIAL COMMUNITIES: A BIOTECHNOLOGY STUDY WITH SINGLE-CELL SEQUENCING
reservedMicroorganisms are the most abundant life forms on Earth, yet an estimated 90-99% remain uncharacterized due to the limitations of traditional culturing methods in laboratory settings.
ROSSI, MARIA
core
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Single-cell genotyping workflow implementing the Single-Cell Printer.
, 2016 (A) The cell suspension is filled into the sterile single-use cartridge. (B) The microwell plate holder is equipped with a camera to automatically determine and adjust for the dispenser offset prior to cell printing (automatic offset compensation, AOC ...
Björn Hackanson (14623) +14 more
core +1 more source
DEVELOPMENT AND APPLICATION OF SINGLE CELL MASS SPECTROMETRY TECHNIQUES FOR NON-ADHERENT CELL ANALYSIS [PDF]
, 2020 Mass spectrometry (MS) analysis of biological samples is traditionally carried out using extractions from large populations of cells, concealing the information from individual cells.
Zhu, Yanlin
core
Diversity and complexity in neural organoids
FEBS Letters, EarlyView.Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley +1 more source