Results 121 to 130 of about 6,472,690 (313)
FEBS Letters, EarlyView.The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi +6 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Single stranded DNA: A new model system for single molecule polymer dynamics [PDF]
, 2011 The study of polymer dynamics has been an active area of research for many years. For nearly two decades, fluorescently-labeled double stranded DNA (dsDNA) has been the model system for studying single molecule polymer dynamics in non-equilibrium ...
Brockman, Christopher A.
core
Single-Molecule Imaging Reveals Topology Dependent Mutual Relaxation of Polymer Chains [PDF]
, 2015 The motion and relaxation of linear and cyclic polymers under entangled conditions are investigated by means of a newly developed single-molecule tracking technique, cumulative-area (CA) tracking.
Serag, Maged F. +2 more
core +1 more source
Fluorescence Microscopy Assay to Measure HIV-1 Capsid Uncoating Kinetics in vitro
Bio-Protocol, 2019 The stability of the HIV-1 capsid and the spatiotemporal control of its disassembly, a process called uncoating, need to be finely tuned for infection to proceed.
Chantal Márquez +6 more
doaj +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
, 2019 Here we report molecular films terminated with diazonium salts moieties at both ends which enables single-molecule contacts between gold and silicon electrodes at open circuit via a radical reaction.
Yan B Vogel (23524978) +7 more
core
Single-Molecule Imaging of Bmp4 Dimerization on Human Periodontal Ligament Cells
, 2012 We expressed bone morphogenetic protein 4 (BMP4) fused with enhanced green fluorescent protein (BMP4-EGFP) in the secretory pathways of producer cells. Fluorescent EGFP was acquired only after we interrupted the transport of BMP4- EGFP by culturing cells
秘心吾;姜昱至;周綠蘋;林俊彬 +1 more
core +1 more source
Pharmacologic hyperstabilisation of the HIV-1 capsid lattice induces capsid failure
eLifeThe HIV-1 capsid has emerged as a tractable target for antiretroviral therapy. Lenacapavir, developed by Gilead Sciences, is the first capsid-targeting drug approved for medical use.
KM Rifat Faysal +12 more
doaj +1 more source
Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis
eLife, 2018 Uncoating of the metastable HIV-1 capsid is a tightly regulated disassembly process required for release of the viral cDNA prior to nuclear import. To understand the intrinsic capsid disassembly pathway and how it can be modulated, we have developed a ...
Chantal L Márquez +10 more
doaj +1 more source