Results 221 to 230 of about 310,423 (261)
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Imaging of Single Organic Molecules in Motion
Science, 2007High-resolution transmission electron microscopy revealed nearly atomically precise images of stepping conformational change and translational motion of single hydrocarbon molecules confined in carbon nanotubes. One or two C12 or C22 alkyl chains were tethered to a carborane end group and then embedded in the nanotubes. Images of the hydrocarbon chains
Masanori, Koshino +5 more
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2014
Single-molecule imaging is a powerful tool to understand workingmechanisms of glycoproteins and glycolipids in cell plasma membranes by overcoming intrinsically stochastic and inhomogeneous properties of biological molecular systems. Here, basic techniques for single-molecule imaging microscopy are described.
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Single-molecule imaging is a powerful tool to understand workingmechanisms of glycoproteins and glycolipids in cell plasma membranes by overcoming intrinsically stochastic and inhomogeneous properties of biological molecular systems. Here, basic techniques for single-molecule imaging microscopy are described.
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Single‐Molecule Imaging of Fluorescent Proteins
2008Single molecule imaging techniques overcome the averaging effects inherent in ensemble measurements and enable characterization of the enormous heterogeneity that exists in biomolecular systems. Though long the domain of a few highly specialized labs, optical imaging of single molecules in living cells is becoming a widely accessible technique.
Adam D, Douglass, Ronald D, Vale
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Improving certainty in single molecule imaging
Current Opinion in Structural Biology, 2017The spatiotemporal organization of biological entities and the complex network of interactions they sponsor has proven challenging to visualize. Modern biophysics has brought a wealth of techniques for probing cellular structure and dynamics of which fluorescence-based single molecule detection has emerged as a powerful tool.
Danial, J. +1 more
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Imaging Single mRNA Molecules in Yeast
2010Yeast cells in an isogenic population do not all display the same phenotypes. To study such variation within a population of cells, we need to perform measurements on each individual cell instead of measurements that average out the behavior of a cell over the entire population.
Hyun, Youk +2 more
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Mechanics and imaging of single DNA molecules
2003We review recent experiments that have revealed mechanical properties of single DNA molecules using advanced manipulation and force sensing techniques(scanning force microscopy (SFM), optical or magnetic tweezers, microneedles). From such measurements, intrinsic relevant parameters (persistence length, stretch modulus) as well as their dependence on ...
M, Hegner, W, Grange
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Electrons for single molecule diffraction and imaging
Ultramicroscopy, 2012We demonstrate the potential of electrons for single molecule diffraction and imaging using C₆₀ molecules confined inside single-walled carbon nanotubes (C₆₀s@SWCNT or peapod) as a model system. A 25 nm diameter electron beam from a field emission gun source is used to record diffraction patterns from individual peapods using imaging plates.
Ke, Ran +3 more
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Single Molecule Imaging of RNA In Situ
2011This protocol describes a method to image individual mRNA molecules in situ. About 50 oligonucleotides complementary to different regions of a target mRNA species are used simultaneously. Each probe is labeled with a single fluorescent moiety. When these probes bind to their target, each mRNA molecule becomes so intensely fluorescent that it can be ...
Mona, Batish, Arjun, Raj, Sanjay, Tyagi
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Combining Single-Molecule Imaging and Single-Channel Electrophysiology
Journal of Molecular Biology, 2015Combining simultaneous single-molecule fluorescence measurements of ion channel conformational change with single-channel electrophysiology would enable a direct link between structure and function. Such methods would help us to create a truly molecular "movie" of how these important biomolecules work.
Eve E. Weatherill, Mark I. Wallace
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Spectral imaging of single molecules
Single Molecules, 2000Using a setup based on fluorescence microscopy we study fluorescence spectra of single chromophores. Being point like emission sources single molecules can be focused to the entrance slit of a spectrometer. Even with narrow slit width the light passes the system with almost no loss.
Hermann Bach, Alois Renn, Urs. P. Wild
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