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Single-molecule imaging of cytoplasmic dynein in vivo

2015
While early fluorescence microscopy experiments employing fluorescent probes afforded snapshots of the cell, the power of live-cell microscopy is required to understand complex dynamics in biological processes. The first successful cloning of green fluorescent protein in the 1990s paved the way for development of approaches that we now utilize for ...
Ananthanarayanan, Vaishnavi   +1 more
openaire   +4 more sources

Single-Molecule Imaging of Endogenous Proteins

Single-molecule imaging is a technique of choice to investigate the dynamic nanoscale organization of proteins of interest, revealing a wealth of new information on how proteins perform their biological function. However, overexpression, which is often used in this context, can alter biological functions.
Malapaka, Anusha   +2 more
openaire   +2 more sources

Single-molecule Imaging in Biosystems

2023
The imaging of living systems plays a key role in understanding biomechanics and overall living systems in depth. This chapter discusses various approaches to and the importance of the use of single-molecule imaging (SMI) in the context of systems biology and describes the technological issues that arise in this setting.
openaire   +1 more source

Cell biochemistry studied by single-molecule imaging

Biochemical Society Transactions, 2006
Over the last decade, there have been remarkable developments in live-cell imaging. We can now readily observe individual protein molecules within living cells and this should contribute to a systems level understanding of biological pathways. Direct observation of single fluorophores enables several types of molecular information to be gathered ...
G I, Mashanov   +3 more
openaire   +2 more sources

Imaging single molecules in motion

Chemical & Engineering News Archive, 1997
Continuous imaging of single molecules of a highly fluorescent compound and a fluorescent-tagged DNA has been achieved by postdoctoral associate Xiao-Hong Xu and chemistry professor Edward S. Yeung of the department of chemistry at Iowa State University, Ames [Science, 275, 1106 (1997)].
openaire   +1 more source

Single mRNA Imaging with Fluorogenic RNA Aptamers and Small‐molecule Fluorophores

Angewandte Chemie - International Edition, 2023
Xing Li
exaly  

Single-Molecule Imaging of Protein Interactions and Dynamics

Annual Review of Analytical Chemistry, 2020
Xiaohong Fang, Tie Xia
exaly  

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