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Single-molecule imaging of cytoplasmic dynein in vivo
2015While early fluorescence microscopy experiments employing fluorescent probes afforded snapshots of the cell, the power of live-cell microscopy is required to understand complex dynamics in biological processes. The first successful cloning of green fluorescent protein in the 1990s paved the way for development of approaches that we now utilize for ...
Ananthanarayanan, Vaishnavi +1 more
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Single-Molecule Imaging of Endogenous Proteins
Single-molecule imaging is a technique of choice to investigate the dynamic nanoscale organization of proteins of interest, revealing a wealth of new information on how proteins perform their biological function. However, overexpression, which is often used in this context, can alter biological functions.Malapaka, Anusha +2 more
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Electrochemiluminescence from Single Molecule to Imaging
Analytical Chemistry, 2023Jinrun Dong, Jiandong Feng
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Single-molecule Imaging in Biosystems
2023The imaging of living systems plays a key role in understanding biomechanics and overall living systems in depth. This chapter discusses various approaches to and the importance of the use of single-molecule imaging (SMI) in the context of systems biology and describes the technological issues that arise in this setting.
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Cell biochemistry studied by single-molecule imaging
Biochemical Society Transactions, 2006Over the last decade, there have been remarkable developments in live-cell imaging. We can now readily observe individual protein molecules within living cells and this should contribute to a systems level understanding of biological pathways. Direct observation of single fluorophores enables several types of molecular information to be gathered ...
G I, Mashanov +3 more
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Imaging single molecules in motion
Chemical & Engineering News Archive, 1997Continuous imaging of single molecules of a highly fluorescent compound and a fluorescent-tagged DNA has been achieved by postdoctoral associate Xiao-Hong Xu and chemistry professor Edward S. Yeung of the department of chemistry at Iowa State University, Ames [Science, 275, 1106 (1997)].
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Single mRNA Imaging with Fluorogenic RNA Aptamers and Small‐molecule Fluorophores
Angewandte Chemie - International Edition, 2023Xing Li
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Single-Molecule Imaging Reveals Translation of mRNAs Localized to Stress Granules
Cell, 2020Jan Eglinger +2 more
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Single-Molecule Imaging of Protein Interactions and Dynamics
Annual Review of Analytical Chemistry, 2020Xiaohong Fang, Tie Xia
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