Results 71 to 80 of about 5,783,756 (283)

TNER: a novel background error suppression method for mutation detection in circulating tumor DNA

BMC Bioinformatics, 2018
Background Ultra-deep next-generation sequencing of circulating tumor DNA (ctDNA) holds great promise as a tool for the early detection of cancer and for monitoring disease progression and therapeutic responses. However, the low abundance of ctDNA in the
Shibing Deng, Maruja Lira, Donghui Huang, Kai Wang, Crystal Valdez, Jennifer Kinong, Paul A. Rejto, Jadwiga Bienkowska, James Hardwick, Tao Xie   +9 more
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

A genetic variation map for chicken with 2.8 million single-nucleotide polymorphisms [PDF]

, 2004
We describe a genetic variation map for the chicken genome containing 2.8 million single-nucleotide polymorphisms (SNPs). This map is based on a comparison of the sequences of three domestic chicken breeds (a broiler, a layer and a Chinese silkie) with ...
Wilson, Richard K, Wei, D, Lan, F, Rothwell, L, Zheng, Weimou, Webber, Caleb, Boardman, Paul E., Ponting, Chris, Yang, Xu, Huang, Xiangang, Wei, Dong Qing, Wei, N, Zheng, Hongkun, Bovenhuis, Henk, Wang, J., Brandström, M, Warren, Wesley, Lin, Wei, Liu, Bin, Chen, Chen, Zhang, Zhenpeng, Zhao, W, Huang, Yanqing, Sun, Yongqiao, Zhao, Y, Hu, SN, Ni, PX, Ni, Peixiang, Hao, Bailin, Gordon, Laurie, Wilson, Richard K., Crooijmans, Richard P. M. A., Xie, Fei, Lan, FD, de Koning, Dirk-Jan, Young, John R, Crooijmans, R.P.M.A., Burt, DW, Cheng, Hans H., Wang, XL, Wahlberg, P, Li, Guoqing, Li, Guangyun, Kindlund, E, Aerts, Andrea, Hao, BL, Lamont, Susan J., Zhou, Jun, H, Li, Ruiqiang, Qi, Qiuhui, van der Poel, Jan, Wang, J, Zeng, CQ, Wang, M, Wang, P, Zhao, Wenming, Li, RQ, Glavina, Tijana, Groenen, Martien, Lamont, Susan, Wang, X, Wang, JJ, Zhou, Yan, Li, Ning, Kindlund, Ellen, Wang, Jian, Overton, IM, Dai, Mmingtao, Morrice, Dvaid, Zhang, Yunze, Rogers, S, Young, John, Li, SG, Hunt, H, Wei, Dong, Zhang, Y, Zhang, X, Zhang, Z, Tong, Wei, Groenen, Martien A M, Yang, Zheng, Wong, Gane Ka-Shu, Wang, Xiaoling, Yu, Yingpu, Liu, B, Burt, David W., Wang, Pei, Tong, W, Li, ST, Zhang, J, Qi, Q, Cheng, HH, Hocking, Paul M, de Koning, DJ, Meng, QS, Land, Fengdi, Li, Songgang, Tang, Haizhou, Wang, MH, Cheng, Hans, He, XM, Ovcharenko, I, Crooijmans, Richard, Morrice, David, van der Poel, Jan J, Walker, Brian, Ye, Chen, Ellegren, Hans, Tammi, Martti T., Yu, Jun, Aerts, A, Kaiser, P, Yu, YP, Wang, Xioaling, Li, Heng, Zhang, Xiaowei, Wilson, SA, Bartly, Neil, Wilson, Stuart A., Shi, Jianping, Dong, Le, Lucas, Susan, He, Ximiao, Hillier, LaDeana, Webber, C, Young, JR, ?, ?, Bartley, Neil, Warren, WC, van der Poel, JJ, Wilson, RK, Walker, Brian A., Li, N, Ovcharenko, Ivan, Li, J, Li, H, Yang, SP, Li, G, Overton, Ian M., Li, D, Zhang, Zengjin, Ran, Longhua, Hillier, LaDeana W., Lamont, SJ, Bartley, N, Qi, QH, Walker, BA, Lucas, S, Stubbs, Lisa, Huang, X, Huang, Y, Burt, David, Andersson, Leif, Wang, Jun, Overton, Ian M; id_orcid, Li, Jun, Crooijmans, Richard P M A, Huang, Xingang, Li, Y, Andersson, Björn, Lin, W, Li, W, Wong, GKS, Brandström, Mikael, Li, S, Young, John R., Li, R, Bovenhuis, H., He, X, van der Poel, J.J., Zhang, XW, Hocking, PM, Ran, LH, Gordon, L, Rogers, Sally L, Tammi, Martti, Zeng, C, Dai, MT, Li, GY, Bumstead, N, He, DD, Liu, B., Bovenhuis, H, Yang, N, Wilson, Stuart, Yang, H, Burt, David W, Yang, Huanming, Stubbs, L, Hubbard, Simon, Hillier, LW, Groenen, M.A.M., Wahlberg, Per, Yang, Z, Kaiser, Pete, Xi, Yan, Hu, Songnian, Yang, X, Zhao, WM, Walker, Brian A, van Hateren, A, Zhang, Jianguo, He, D, Lamont, Susan J, Hocking, Paul, Li, YZ, Hu, S, Zhang, Yong, Rothwell, Lisa, Huang, YQ, Li, Wenjie, Wong, GK, Zhang, JG, Zhang, ZJ, Zhang, ZP, Zhang, Jingjing, Li, Shengting, Wong, G.K., Rogers, Sally, Wang, Jianjun, Hunt, Henry, Cheng, Hans H, Zhang, YZ, Li, Guangyuan, Ponting, CP, Gunnarsson, U, Hubbard, Simon J, Zhao, Yiqiang, Tammi, Martti T, Glavina, T, Wong, Gane, Ruan, J, Hubbard, SJ, Yang, Shiaw-Pyng, Boardman, Paul E, Xie, F, Hao, B, Yang, Shuaw-Pyng, Overton, Ian, Boardman, Paul, Yu, J, Wilson, Richard, Law, Andy, Hillier, Ladeana W, Zhang, Zhi-Yong, Zhou, Y, Bumstead, Nat, Ran, L, Hocking, Paul M., Zheng, HK, Zhou, J, Meng, Q, Zhou, H, Lan, Fengdi, Ye, Jia, Tammi, MT, van Hateren, Andy, Kaufman, J, Ponting, Chris P., Dai, Mingtao, He, Dandan, Li, Dawei, Li, DW, Huang, XG, Consortium, International Chicken Polymorphism Map, Chen, Jie, Wang, Miaoheng, Warren, Wesley C, Kaufman, Jim, Yu, Y, Law, A, Ye, C, Shi, JP, Morrice, D, Zhang, JJ, Warren, Wesley C., Xi, Y, Groenen, Martien A. M., Ye, J, Yang, Ning, Wei, Ning, Ellegren, H, Tang, H, Wilson, Stuart A, Ponting, Chris; id_orcid, Andersson, B, Zheng, WM, Zheng, H, Shi, J, Groenen, MA, Gunnarsson, Ulrika, Zeng, Changqing, Chen, J, Ruan, Jue, Li, Yuanzhe, Boardman, PE, Sun, YQ, Ponting, Chris P, Ni, P, Chen, C, Zheng, W, Andersson, L, Poel, J.J., van der, Dong, Wei, Crooijmans, RP, Zhao, YQ, van der Poel, Jan J., Hubbard, Simon; id_orcid, Overton, Ian M, Zhou, Huaijun, Li, WJ, Wang, Jing, Dong, W, Hubbard, Simon J., Meng, Qingshun, Li, GQ, Dong, L, Sun, Y, Dai, M   +319 more
core   +1 more source

Genotyping single point mutations in rd1 and rd8 mice using melting curve analysis of qPCR fragments

Scientific Reports
PCR is tolerant to single nucleotide mismatches. Therefore, genotyping of point mutations by PCR requires special conditions for the amplification of allele-specific PCR fragments.
Melanie E. Schwämmle, Felicitas Bucher, Günther Schlunck, Gottfried Martin   +3 more
doaj   +1 more source

Prenatal Diagnosis of 17p11.2 Copy Number Abnormalities Associated With Smith–Magenis and Potocki–Lupski Syndromes in Fetuses

Frontiers in Genetics, 2021
Smith-Magenis syndrome and Potocki-Lupski syndrome are rare autosomal dominant diseases. Although clinical phenotypes of adults and children have been reported, fetal ultrasonic phenotypes are rarely reported.
Meiying Cai, Xianguo Fu, Liangpu Xu, Na Lin, Hailong Huang   +4 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

Whole-genome sequencing of phenotypically distinct inflammatory breast cancers reveals similar genomic alterations to non-inflammatory breast cancers

Genome Medicine, 2021
Background Inflammatory breast cancer (IBC) has a highly invasive and metastatic phenotype. However, little is known about its genetic drivers. To address this, we report the largest cohort of whole-genome sequencing (WGS) of IBC cases.
Xiaotong Li, Sushant Kumar, Arif Harmanci, Shantao Li, Robert R. Kitchen, Yan Zhang, Vikram B. Wali, Sangeetha M. Reddy, Wendy A. Woodward, James M. Reuben, Joel Rozowsky, Christos Hatzis, Naoto T. Ueno, Savitri Krishnamurthy, Lajos Pusztai, Mark Gerstein   +15 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Single-variant Association Analyses

, 2018
Code for and results from the single-variant association analyses with ...
Taylor, Margaret R., Sadowsky, Michael J., Tiffin, Peter, Matthew Nelson (1902790), Abdelaal Shameldsin (5857106), Liana T. Burghardt (3261864), Peter Tiffin (104127), Abou-Shanab, Reda A.l., Reda A.l. Abou-Shanab (5857103), Margaret R. Taylor (2649307), Epstein, Brendan, Burghardt, Liana T., Shameldsin, Abdelaal, Brendan Epstein (147035), Joseph Guhlin (3864577), Nelson, Matthew, Guhlin, Joseph, Michael J. Sadowsky (147063)   +17 more
core   +1 more source

Optimizing CRISPR/Cas9 Editing of Repetitive Single Nucleotide Variants

Frontiers in Genome Editing, 2022
CRISPR/Cas9, base editors and prime editors comprise the contemporary genome editing toolbox. Many studies have optimized the use of CRISPR/Cas9, as the original CRISPR genome editing system, in substituting single nucleotides by homology directed repair (HDR), although this remains challenging.
Inga Usher, Lorena Ligammari, Sara Ahrabi, Emily Hepburn, Calum Connolly, Gareth L. Bond, Adrienne M. Flanagan, Adrienne M. Flanagan, Lucia Cottone   +8 more
openaire   +4 more sources