Results 191 to 200 of about 2,082,962 (313)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Crystal structures of Salmonella enterica FraB deglycase reveal a conformational heterodimer with remarkable structural plasticity at the active site. [PDF]

Protein Sci
Zakharova K, Law JD, Gao Y, Kovvali S, Wysocki VH, Gopalan V, Bell CE.   +6 more
europepmc   +1 more source

Dynamic Active Sites In Situ Formed in Metal Nanoparticle Reshaping under Reaction Conditions [PDF]

Xiaoyan Li, Pengfei Ou, Xinyi Duan, Lei Ying, Jun Meng, Beien Zhu, Yi Gao   +6 more
openalex   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

The details and entropy demons in a transmembrane allosteric machine. [PDF]

PLoS Biol
Luisi BF.
europepmc   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Cohesin drives chromatin scanning during the RAD51-mediated homology search. [PDF]

Science
Marin-Gonzalez A, Rybczynski AT, Nilavar NM, Nguyen D, Li AG, Karwacki-Neisius V, Zou RS, Avilés-Vázquez FJ, Kanemaki MT, Scully R, Ha T.   +10 more
europepmc   +1 more source

Report of an investigation into deterioration of the Plutonium Fuel Form Fabrication Facility (PuFF) at the DOE Savannah River Site

, 1991
USDOE Office of Nuclear Safety, Washington, DC (United States)
openalex   +2 more sources

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Recent Advances in the Design and Structure-Activity Relationships of Oxygen Evolution Catalysts for Alkaline Water Electrolysis. [PDF]

Molecules
Wang L, Liu X, Lai C, Liu J, Wang W, Wang X, Bo X, Cheng T, Li J, Wang Z, Lu X.   +10 more
europepmc   +1 more source