Results 101 to 110 of about 372,530 (379)
Comparison of Ultrafiltration and Size-Exclusion Chromatography Performances for Proteins Desalination [PDF]
, 2014 Protein purification is a part of biotechnology. Its application can vary from laboratory researches to industrial production. Nowadays there are a lot of different methods of purification, which involve chromatographic and membrane-based separations.
Akhmetova, Arina
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A high-affinity antibody against the CSP N-terminal domain lacks Plasmodium falciparum inhibitory activity [PDF]
, 2020 Malaria is a global health concern and research efforts are ongoing to develop a superior vaccine to RTS,S/AS01. To guide immunogen design, we seek a comprehensive understanding of the protective humoral response against Plasmodium falciparum ...
Bosch, A. +15 more
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Multicolour fluorescence-detection size-exclusion chromatography for structural genomics of membrane multiprotein complexes. [PDF]
PLoS ONE, 2013 Many interesting and important membrane proteins are hetero-oligomeric. However, besides naturally abundant examples, the structures of relatively few such complexes are known.
David Parcej +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
Mercury and selenium binding biomolecules in terrestrial mammals (Cervus elaphus and Sus scrofa) from a mercury exposed area [PDF]
, 2017 Acknowledgements The authors are grateful to Junta de Comunidades de Castilla-La Mancha (PCC-05-004-2, PAI06-0094, PCI-08-0096, PEII09-0032-5329) and the Ministerio de Economía y Competitividad (CTQ2013-48411-P) for financial support. M.J.
Fariñas, N. Rodríguez +5 more
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Molecules, 2017 This paper deals with the use of tetrabutylammonium fluoride/dimethylsulfoxide (TBAF/DMSO) to characterize the molar mass distribution of non-derivatized cellulosic samples by size exclusion chromatography (SEC).
Jérémy Rebière +3 more
doaj +1 more source
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Gel filtration chromatography [PDF]
, 2011 Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted.
Cummins, Phil +2 more
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Viscous fingering of miscible slices [PDF]
, 2005 Viscous fingering of a miscible high viscosity slice of fluid displaced by a lower viscosity fluid is studied in porous media by direct numerical simulations of Darcy's law coupled to the evolution equation for the concentration of a solute controlling ...
Bertho, Yann +2 more
core +8 more sources
BMC Research Notes, 2009 Background We previously developed a set of rationally designed mutant MICA protein ligands for the NKG2D immunoreceptor in which MICA was mutated at residues that do not contact NKG2D.
Austin Chad R +5 more
doaj +1 more source