Results 321 to 330 of about 372,530 (379)

Extracellular-Vesicle Isolation from Different Biological Fluids by Size-Exclusion Chromatography.

Current protocols in stem cell biology, 2019
This unit describes how to isolate extracellular vesicles (EVs) from different biological fluids using size-exclusion chromatography (SEC) and how to prepare your starting sample and the EV product for downstream applications.
M. Monguió-Tortajada, M. Morón-Font, Ana Gámez-Valero, Laura Carreras-Planella, F. Borràs, M. Franquesa   +5 more
semanticscholar   +1 more source

Optimizing Size Exclusion Chromatography for Extracellular Vesicle Enrichment and Proteomic Analysis from Clinically Relevant Samples

Proteomics, 2019
The field of extracellular vesicle (EV) research has rapidly expanded in recent years, with particular interest in their potential as circulating biomarkers.
R. Lane, D. Korbie, M. Trau, M. Hill
semanticscholar   +1 more source

Challenges of Size-Exclusion Chromatography for the Analysis of Bottlebrush Polymers

, 2020
The establishment of structure–function relationships requires the accurate characterization of the materials being studied.
D. Walsh, Matthew A. Wade, S. Rogers, Damien Guironnet   +3 more
semanticscholar   +1 more source

Surfactant-aided size exclusion chromatography

Journal of Chromatography B, 2004
The flexibility and selectivity of size exclusion chromatography (SEC) for protein purification can be modified by adding non-ionic micelle-forming surfactants to the mobile phase. The micelles exclude proteins from a liquid phase similar to the exclusion effect of the polymer fibers of the size exclusion resin.
Horneman, D.A., Wolbers, M., Zomerdijk, M., Ottens, M., Keurentjes, J.T.F., Wielen, van der, L.A.M.   +5 more
openaire   +2 more sources

Aqueous size exclusion chromatography

Analytica Chimica Acta, 1989
Part I. Separation Mechanisms. Size exclusion parameters (M.E. Himmel, P.G. Squire). Partitioning: Hydrophobic interactions (M. Janado). Electrostatic effects (P.L. Dubin). Exclusion chromatography of inorganic compounds (M. Shibukawa, N. Ohta). II. Characterization of Stationary Phases. Pore size distributions (L. Hagel). Structural analysis of porous
P.L. Dubin, D.Thorburn Burns
openaire   +1 more source

Quantitative Determination of Protein-Ligand Affinity by Size Exclusion Chromatography Directly Coupled to High-Resolution Native Mass Spectrometry.

Analytical Chemistry, 2018
High throughput protein-ligand interaction screening assays employing mass spectrometric detection are widely used in early stage drug discovery. Mass spectrometry-based screening approaches employ a target protein added to a pool of small-molecule ...
Chengfeng Ren, A. Bailey, Erica C Vanderporten, A. Oh, W. Phung, Melinda M. Mulvihill, S. Harris, Yichin Liu, Guanghui Han, W. Sandoval   +9 more
semanticscholar   +1 more source

Size exclusion chromatography

Analytical Chemistry, 1990
H G, Barth, B E, Boyes
openaire   +4 more sources

Longitudinal diffusion in size-exclusion chromatography: a stop-flow size-exclusion chromatography study

Journal of Chromatography A, 2001
Band broadening in size-exclusion chromatography (SEC) has an adverse effect upon calculated molecular mass averages, distributions, and dilute solution data generated using single- and multi-detector systems. In the past, the longitudinal diffusion contribution to band broadening in SEC has been considered negligible.
openaire   +2 more sources

Size Exclusion Chromatography System

2001
Abstract : Instrumentation was purchased as planned set up, and used to support the ongoing ARO grant "Elastomeric Nanocomposites Based On Sol-Gel Reactions of Silicon Alkoxides In lonomeric Derivatives Of Finely-Architectured Block Copolymers". Research results are presented in the attached document.
Robson F. Storey, Kenneth A. Mauritz
openaire   +1 more source

Aqueous Size Exclusion Chromatography

1999
The determination of molecular weight (MW) averages and molecular weight distributions (MWD) of water-soluble polymers, such as nonionic and ionic synthetic polymers, proteins and peptides, by aqueous SEC sometimes encounters difficulties because of non-size exclusion effects.
Sadao Mori, Howard G. Barth
openaire   +1 more source