Results 71 to 80 of about 372,530 (379)

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

open access: yesFEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li   +2 more
wiley   +1 more source

SEC-MALS characterization of natural rubber: Study of phenomena behind the abnormal elution profile [PDF]

open access: yes, 2008
Natura/ and synthetic poly(cis-1,4-isoprene) were characterized by size exclusion chromatography coupled with an online multiangle light scattering detector (SEC-MALS) (Fig. 1).
Kim, Chandy   +4 more
core  

The trouble with ODE : polymerization during nanocrystal synthesis [PDF]

open access: yes, 2019
1-Octadecene is a widely used solvent for high temperature nanocrystal synthesis (120-320 degrees C). Here, we show that 1-octadecene spontaneously polymerizes under these conditions, and the resulting poly(1-octadecene) has a comparable solubility and ...
Bennett, Ellie   +4 more
core   +2 more sources

Comparison of an Optimized Ultracentrifugation Method versus Size-Exclusion Chromatography for Isolation of Exosomes from Human Serum.

open access: yesJournal of Proteome Research, 2018
Exosomes are nanosized vesicles that are abundant in biological fluids. In recent years, exosomes have attracted increasing attention as their cargo may provide promising biomarkers for the early diagnosis of and therapy for many diseases, such as cancer.
Mingrui An   +3 more
semanticscholar   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

open access: yesFEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan   +5 more
wiley   +1 more source

Analysis Using Size Exclusion Chromatography of poly(N-isopropyl acrylamide) using Methanol as an Eluent [PDF]

open access: yes, 2017
YesSize Exclusion Chromatography is traditionally carried out in either aqueous or non-polar solvents. A system to present molar mass distributions of polymers using methanol as a mobile phase is presented.
Amu   +43 more
core   +1 more source

A cryostat-based frozen section method to increase the yield of extracellular vesicles extracted from different tissues

open access: yesBioTechniques, 2022
Extracellular vesicles (EVs) are small vesicles mediating intercellular communications that have been widely used in disease diagnosis. Extracting EVs from tissues is of great importance, but current approaches are finite and the EV yield is limited ...
Shuangzhu Yang   +8 more
doaj   +1 more source

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

open access: yesMolecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou   +5 more
wiley   +1 more source

Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies

open access: yesScientific Reports, 2017
Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-
Birke J. Benedikter   +11 more
semanticscholar   +1 more source

Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method

open access: yesJurnal Natural, 2021
Vannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg
HARIYANTI HARIYANTI, HANIFAH RAHMI
doaj   +1 more source

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