Results 31 to 40 of about 178,510 (288)
Directive Speech Act in The Movie “Sleeping Beauty”
Humaniora, 2013 Pragmatics is one of linguistics studies that is quite attractive to learn more about. There are many aspects of pragmatics; one of them is dealing with speech acts. Speech acts consist of many categories; one of them is directive speech act.
Muhartoyo Muhartoyo, Keilly Kristani
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Comparison of lentiviral and sleeping beauty mediated αβ T cell receptor gene transfer.
PLoS ONE, 2013 Transfer of tumour antigen-specific receptors to T cells requires efficient delivery and integration of transgenes, and currently most clinical studies are using gamma retroviral or lentiviral systems.
Anne-Christine Field +9 more
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Sleeping Beauty transposon integrates into non-TA dinucleotides
Mobile DNA, 2018 Background Sleeping Beauty transposon (SB) has become an increasingly important genetic tool for generating mutations in vertebrate cells. It is widely thought that SB exclusively integrates into TA dinucleotides.
Yabin Guo, Yin Zhang, Kaishun Hu
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Rediscovering Forgotten Research: Sleeping Beauties at the University of Waterloo [PDF]
Journal of Information Science Theory and Practice, 2018 An academic article is normally cited within a few years of publication, after which interest falls off as the research field moves on. However, an article is sometimes ignored for many years only to attract interest after a long period of dormancy. Such
Demaine, Jeffrey
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RNA-guided retargeting of Sleeping Beauty transposition in human cells
eLife, 2020 An ideal tool for gene therapy would enable efficient gene integration at predetermined sites in the human genome. Here we demonstrate biased genome-wide integration of the Sleeping Beauty (SB) transposon by combining it with components of the CRISPR ...
Adrian Kovač +5 more
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Molecular Oncology, EarlyView.Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra +10 more
wiley +1 more source
Efficient Sleeping Beauty DNA Transposition From DNA Minicircles
Molecular Therapy: Nucleic Acids, 2013 DNA transposon-based vectors have emerged as new potential delivery tools in therapeutic gene transfer. Such vectors are now showing promise in hematopoietic stem cells and primary human T cells, and clinical trials with transposon-engineered cells are ...
Nynne Sharma +5 more
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Molecular Oncology, EarlyView.Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova +25 more
wiley +1 more source
PolythesisThis article examines several medieval adaptations of the Sleeping Beauty tale type, including Perceforest, Blandin de Cornoalha, Roman de Bleris, and Frayre de Joy e Sorde Plazer.
Benedetta Viscidi
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