Results 141 to 150 of about 381,109 (217)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Three distinct trajectories of red blood cell distribution width and their significant associations with mortality in sepsis patients: a group-based trajectory modeling study with validation. [PDF]

Front Med (Lausanne)
Cai L, Hua Y, Lu W, Bing H, Gao Q, Zhang W.   +5 more
europepmc   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Net release of CO₂ from thawing permafrost soil carbon predicted to occur earlier in this century. [PDF]

Sci Adv
Xi Y, Ciais P, Zhu D, Qiu C, Zhang Y, Peng S, Bowring SPK, Goll DS, Friedlingstein P, Hugelius G.   +9 more
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Evaluation of tumor localization accuracy on fast ring-gantry cone-beam computed tomography using patient-specific breathing curves and a dynamic anthropomorphic thorax phantom. [PDF]

Phys Imaging Radiat Oncol
Daenen LHBA, Lustermans D, Stassen THA, Szkitsak J, Abdulrahim R, Goossens J, Rishmawi G, Canters R, Rinaldi I, Verhaegen F, Fonseca GP.   +10 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Case Report: Tocilizumab in pediatric febrile infection-related epilepsy syndrome. [PDF]

Front Immunol
Pang L, Liu X, Sun S, Chen F, Lu H, Du Y, Li X.   +6 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Epilepsy associated with <i>SYNGAP1</i> gene variants: clinical features of six cases and a literature review. [PDF]

Front Pediatr
Zhang W, Wang Y, Xu K, Wang L, Wang Y, Chen G, Ma Y.   +6 more
europepmc   +1 more source