Results 51 to 60 of about 932 (214)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Spatial and single‐nuclei transcriptomics reveals idiosyncratic and generic patterns in papillary and anaplastic thyroid cancers

Molecular Oncology, EarlyView.
Matched spatial transcriptomics and single‐nuclei RNA‐seq were generated for anaplastic and BRAFV600E papillary thyroid cancers revealing generic and tumor‐specific states occurring in cancer cells and in the tumor microenvironment. In this context, cancer dedifferentiation mirrored organoid maturation through ordered thyroid marker gain/loss ...
Adrien Tourneur, Joel Rodrigues Vitória, Manuel Saiselet, Ligia Craciun, Denis Larsimont, Anne Lefort, Frédérick Libert, Carine Maenhaut, Sabine Costagliola, Maxime Tarabichi, Mirian Romitti, Vincent Detours   +11 more
wiley   +1 more source

A hybrid simple exponential smoothing-barnacles mating optimization approach for parameter estimation: Enhancing COVID-19 forecasting in Malaysia

MethodsX
Single or simple exponential smoothing (SES) is a time series forecasting model popular among researchers due to its simplicity and ease of use. SES only requires one smoothing parameter, alpha, to control how quickly the influence of past observations ...
Azlan Abdul Aziz, Zuriani Mustaffa, Suzilah Ismail, Nor Azriani Mohamad Nor, Nurin Qistina Mohamad Fozi   +4 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

A Parameter Adaptive Method for Image Smoothing

Tsinghua Science and Technology
Edge is the key information in the process of image smoothing. Some edges, especially the weak edges, are difficult to maintain, which result in the local area being over-smoothed. For the protection of weak edges, we propose an image smoothing algorithm
Suwei Wang, Xiang Ma, Xuemei Li
doaj   +1 more source

Cross-Validation, Information Theory, or Maximum Likelihood? A Comparison of Tuning Methods for Penalized Splines

Stats, 2021
Functional data analysis techniques, such as penalized splines, have become common tools used in a variety of applied research settings. Penalized spline estimators are frequently used in applied research to estimate unknown functions from noisy data ...
Lauren N. Berry, Nathaniel E. Helwig
doaj   +1 more source

Approximate Interpolation with Applications to Selecting Smoothing Parameters

Numerische Mathematik, 2005
An approximation problem can be shortly stated as follows: for a finite set \(X\) of points situated in a bounded set \(\Omega\) and a corresponding data values of an unknown function \(f \in C(\Omega)\), a function \(s_{f,X} \in C(\Omega)\) to produce a good approximation is required.
Holger Wendland, Christian Rieger
openaire   +3 more sources

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

COMPUTER-ASSISTED CHOICE OF SMOOTHING PARAMETER IN KERNEL METHODS APPLIED IN ECONOMIC ANALYSES

Quantitative Methods in Economics, 2014
In the kernel method, it is necessary to determine the value of the smoothing parameter. Not without significance is the fact of using the objectivity in the selection of this parameter and a certain automation of the selection procedure, which is ...
Aleksandra Baszczyńska
doaj