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Systematic and taxonomic revision of the genus Austrocactus (Cactaceae) based on morphology and genome wide SNP‐data

TAXON, EarlyView.
Abstract The post‐Miocene climatic history of arid environments in South America has been identified as a key driver of dispersal and diversification, particularly among plant groups such as Cactaceae. Despite their iconic status, many cactus genera remain poorly understood, and comprehensive taxonomic and systematic revisions using morphological and ...
Tim Böhnert, Felix F. Merklinger, Elisabeth Sarnes, Norbert Sarnes, Roberto Kiesling, Maximilian Weigend, Federico Luebert   +6 more
wiley   +1 more source

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Techniques patents for SNP genotyping

Pharmacogenomics, 2003
Single nucleotide polymorphisms (SNPs) are the most abundant form of genetic variation in the human genome, accounting for more than 90% of all differences between individuals. Many complex phenotypes in humans have a significant genetic component and most of the variability is therefore likely to stem from differences in patterns of SNPs.
Richard M. Twyman, Sandy B. Primrose
openaire   +3 more sources

Amplifluor-Based SNP Genotyping

2023
Amplifluor, a genotyping system used to analyze single nucleotide polymorphisms (SNPs), is supplied by Merck-Millipore. Amplifluor is based on polymerase chain reaction (PCR) with two competing allele-specific primers and a SNP specific common reverse primer.
Manmode Darpan, Mohanrao, Senapathy, Senthilvel, Yarabapani Rushwanth, Reddy, Chippa Anil, Kumar, Palchamy, Kadirvel   +4 more
openaire   +2 more sources

Pyrosequencing for SNP Genotyping

2009
Pyrosequencing is a real-time DNA sequencing method. It is based on the transformation of pyrophosphates, released during DNA elongation by DNA polymerase, into measurable light. During DNA elongation, a single pyrophosphate molecule is released following incorporation of a single nucleotide.
Jose Luis Royo, José Jorge Galán
openaire   +3 more sources

Genotyping SNPs With the LightCycler

2004
Here, a single nucleotide polymorphism typing methodology is described based on polymerase chain reaction monitoring, in real time, of fluorescently labeled amplified products using the LightCycler. The main advantages of the system are the time required for the analysis (about 20 min), combined with the robustness, accuracy, and the sensitivity of the
Maria Victoria Lareu, Clara Ruiz-Ponte
openaire   +3 more sources

A New SNP Genotyping Technology by Target SNP-Seq

2023
In order to promote the widespread application of single-nucleotide polymorphism (SNP)-based genotyping, a new method was developed and called target SNP-seq which combined the advantages of multiplex polymerase chain reaction (PCR) amplification and high throughput sequencing on Illumina X Ten platform.
Jian, Zhang, Jingjing, Yang, Changlong, Wen   +2 more
openaire   +2 more sources

SNP Genotyping by Heteroduplex Analysis

2014
Heteroduplex-based genotyping methods have proven to be technologically effective and economically efficient for low- to medium-range throughput single-nucleotide polymorphism (SNP) determination. In this chapter we describe two protocols that were successfully applied for SNP detection and haplotype analysis of candidate genes in association studies ...
Verónica Viviana Lia, Verónica Viviana Lia, Verónica Viviana Lia, Corina M. Fusari, Corina M. Fusari, Andrea F. Puebla, Norma Paniego, Norma Paniego   +7 more
openaire   +3 more sources

Hypertension and SNP Genotyping in Antihypertensive Treatment [PDF]

Cardiovascular Toxicology, 2005
Hypertension is prevalent, affecting approx 20--25% of the adult population in the Western world. Primary hypertension is a multifactorial, complex disorder where many genes and genetic variants are assumed to interact with environmental factors in order to produce the specific blood pressure level for a given individual.
Ulrika Liljedahl, Lisa Kurland, Lars Lind   +2 more
openaire   +2 more sources

SNP Genotyping Technologies

2013
In the recent years, single nucleotide polymorphism (SNP) markers have emerged as the marker technology of choice for plant genetics and breeding applications. Besides the efficient technologies available for SNP discovery even in complex genomes, one of the main reasons for this is the availability of high-throughput platforms for multiplexed SNP ...
Studer, Bruno, Kölliker, Roland
openaire   +2 more sources