Results 291 to 300 of about 120,949 (314)
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SNP Genotyping: The KASP Assay
2014The KASP genotyping assay utilizes a unique form of competitive allele-specific PCR combined with a novel, homogeneous, fluorescence-based reporting system for the identification and measurement of genetic variation occurring at the nucleotide level to detect single nucleotide polymorphisms (SNPs) or inserts and deletions (InDels).
John Holme, Jeffrey Anthony, Chunlin He
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SNP marker detection and genotyping in tilapia
Molecular Ecology Resources, 2012AbstractWe have generated a unique resource consisting of nearly 175 000 short contig sequences and 3569 SNP markers from the widely cultured GIFT (Genetically Improved Farmed Tilapia) strain of Nile tilapia (Oreochromis niloticus). In total, 384 SNPs were selected to monitor the wider applicability of the SNPs by genotyping tilapia individuals from ...
van Bers, N.E.M. +4 more
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The TaqMan Method for SNP Genotyping
2009Single nucleotide polymorphisms (SNPs) are common DNA sequence variations that occur at single bases within the genome. SNPs have been instrumental in elucidating the genetic basis of common, complex diseases using genome-wide association studies, candidate gene case-control association studies, and genome-wide linkage analyses.
Gong Qing Shen +3 more
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Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2005
In this review we describe the principles, protocols, and applications of two commercially available SNP genotyping platforms, the TaqMan SNP Genotyping Assays and the SNPlex Genotyping System. Combined, these two technologies meet the requirements of multiple SNP applications in genetics research and pharmacogenetics.
Michael D. Rhodes +3 more
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In this review we describe the principles, protocols, and applications of two commercially available SNP genotyping platforms, the TaqMan SNP Genotyping Assays and the SNPlex Genotyping System. Combined, these two technologies meet the requirements of multiple SNP applications in genetics research and pharmacogenetics.
Michael D. Rhodes +3 more
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SNP genotype calling with MapReduce [PDF]
Proceedings of third international workshop on MapReduce and its Applications Date, 2012 Genotype measurement is a key step in genome-wide association studies -- those studies that aim to uncover the underlying genetic causes of physical traits, including disease. The leading technology for measuring genotypes is the SNP microarray, where hundreds of thousands of genetic variants are interrogated simultaneously.
Gianluigi Zanetti +2 more
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2007
The identification of genes affecting complex traits (i.e., biological traits affected by several genetic and environmental factors) is a very difficult and challenging task (1, 2, 3). For many complex traits, the observable variation between individuals is quantitative; hence, loci affecting such traits are generally termed quantitative trait loci ...
Jun Wang +3 more
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The identification of genes affecting complex traits (i.e., biological traits affected by several genetic and environmental factors) is a very difficult and challenging task (1, 2, 3). For many complex traits, the observable variation between individuals is quantitative; hence, loci affecting such traits are generally termed quantitative trait loci ...
Jun Wang +3 more
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Allele-Specific PCR in SNP Genotyping
2009The increasing need for large-scale genotyping applications of single nucleotide polymorphisms (SNPs) in model and nonmodel organisms requires the development of low-cost technologies accessible to minimally equipped laboratories. The method presented here allows efficient discrimination of SNPs by allele-specific PCR in a single reaction with standard
Gaudet Muriel +3 more
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Isothermal Amplification Methods for the SNP Genotyping
Current Molecular Medicine, 2019The demands for genotyping techniques with acceptable precision, accuracy, cost-effectiveness in high throughput formats made driving forces for continuous development of novel technologies. A wide range of mutation detection techniques based on polymerase chain reaction (PCR) have been introduced.
Fatemeh Heidari Sharafdarkolaee +3 more
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SNP Genotyping: Technologies and Biomedical Applications
Annual Review of Biomedical Engineering, 2007Single nucleotide polymorphisms (SNPs) are the most frequently occurring genetic variation in the human genome, with the total number of SNPs reported in public SNP databases currently exceeding 9 million. SNPs are important markers in many studies that link sequence variations to phenotypic changes; such studies are expected to advance the ...
Sobin Kim, Ashish Misra
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A comprehensive evaluation of SNP genotype imputation
Human Genetics, 2008Genome-wide association studies have contributed significantly to the genetic dissection of complex diseases. In order to increase the power of existing marker sets even further, methods have been proposed to predict individual genotypes at un-typed loci from other marker sets by imputation, usually employing HapMap data as a reference.
David Ellinghaus +4 more
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