Results 141 to 150 of about 20,143,172 (287)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Benchmarking Machine Learning for Sentiment Analysis: A Case Study of News Articles in Multiple Languages

open access: yesIJCoL
Sentiment analysis is the field of study that analyzes people’s opinions and sentiments towards entities such as products, services and organizations. Brand reputation analysis, competitive intelligence and social network analysis are just a few areas ...
Roberto Zanoli   +4 more
doaj  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

EmoTweetID: A dataset of Indonesian tweets for emotion classification and word embedding construction. [PDF]

open access: yesData Brief
Nugroho KS   +6 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Semantic spaces revisited: investigating the performance of auto-annotation and semantic retrieval using semantic spaces

open access: yes, 2008
Semantic spaces encode similarity relationships between objects as a function of position in a mathematical space. This paper discusses three different formulations for building semantic spaces which allow the automatic-annotation and semantic retrieval ...
Lewis, Paul   +3 more
core   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

open access: yesFEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta   +7 more
wiley   +1 more source

Decide less, communicate more: On the construct validity of end-to-end fact-checking in medicine. [PDF]

open access: yesFind ACL ACL
Joseph S   +8 more
europepmc   +1 more source

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