Development and validation of prediction models for predicting social care strengths and vulnerability in older people: Cohort study using routine data in Adult Social Care. [PDF]
Clarkson P +7 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The compressed workweek in public health and social care - is it sustainable? [PDF]
Matre D.
europepmc +1 more source
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Interprofessional Competence of Health and Social Care Professionals Working in Specialized Palliative Care: A Cross-Sectional Study. [PDF]
Kesonen P +4 more
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Listen up! Adult care leavers speak out : the views of 310 care leavers aged 17-78
Listen Up! Adult Care Leavers Speak Out is a ground-breaking report. Based on a survey of 310 care leavers aged from 17 to 78 that took place in the autumn of 2009, it brings together for the first time a range of data that begins to reveal the ...
Duncalf, Zachari
core
Frameworks to Evaluate the Quality of Public Involvement in Health and Social Care Research: A Scoping Review. [PDF]
Hutton R +5 more
europepmc +1 more source

