Results 51 to 60 of about 15,242,158 (222)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Building an industry of choice: service quality, workforce capacity and consumer-centred funding in disability care [PDF]

, 2013
Disability services in Australia are undergoing large-scale, systemic reform.  In recent years, to improve outcomes for people with disabilities and the efficiency of services, Commonwealth, State and Territory agencies have developed and trialled a
Natasha Cortis
core  

Introducing social pedagogy into Scottish residential child care : an evaluation of the Sycamore Services social pedagogy training programme [PDF]

, 2009
This report provides an introduction to the approach to child care known as social pedagogy and an evaluation of a social pedagogy training programme delivered to a group of child care staff employed in Sycamore Services.
Milligan, Ian
core   +3 more sources

If health organisations and staff engage in research, does healthcare improve? Strengthening the evidence base through systematic reviews

Health Research Policy and Systems
Background There is an often-held assumption that the engagement of clinicians and healthcare organizations in research improves healthcare performance at various levels.
Annette Boaz, Belinda Goodenough, Stephen Hanney, Bryony Soper   +3 more
doaj   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Stage 1 development of a patient-reported experience measure (PREM) for chronic obstructive pulmonary disease (COPD)

npj Primary Care Respiratory Medicine, 2017
Chronic lung disease: Giving importance to patients’ emotions An exploration of patient perceptions of living with chronic lung disease will help develop a new patient reported experience scale. Healthcare services are aiming to provide effective patient-
Susan Walker, Sharon Andrew, Matthew Hodson, C. Michael Roberts   +3 more
doaj   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

Facilitators and challenges to implementing a researcher-in-residence model to build research capacity in adult social care

Health Research Policy and Systems
Background Adult social care in England has long lacked the research infrastructure and capacity common in health, limiting evidence-informed improvement. The Kent Research Partnership (KRP) implemented a dual, bi-directional Researcher-in-Residence (RiR)
Nick Smith, Alison Charles, Vanessa Abrahamson, Rasa Mikelyte, Olivia Trapp, Wenjing Zhang, Ferhana Hashem, John Potts, Georgina Walton, Ann-Marie Towers   +9 more
doaj   +1 more source