Results 131 to 140 of about 133,886 (308)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

View of construction on the AMDC, 2013

open access: yes, 2013
View of construction on the Advanced Manufacturing and Design Centre (AMDC) from Burwood Road, Hawthorn Campus.
Swinburne University of Technology
core  

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

Perceptions of acculturation and social identity construction among three Taiwanese/Chinese migrants in Australia

open access: yes, 2009
In recent years, increasing numbers of Chinese migrants have come to Australia to study or to live. In doing so, they have entered a new cultural space. They are faced with many challenges, not only to do with study experience, workplace experience and
Lo, Shu-Fen (Michelle)
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Expert Estimates of the Potential of AR, VR, BIM and AI Technologies to Support Sustainable Construction 4.0 in Slovak Construction Sector

open access: yesSlovak Journal of Civil Engineering
This paper presents the findings of theoretical and empirical research in the area of sustainable Construction 4.0. In the theoretical part of the article, the findings of the potential of BIM technology to support the development of sustainable ...
Porubčinová Martina   +2 more
doaj   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

A reflexive capability model for sustainable e-business environments in construction supply chain

open access: yes, 2006
Despite the increasing significance of e-business worldwide and construction market leaders developing innovative e-business applications, the widespread uptake in the Australian construction industry is lagging.
London, Kerry (R19003)   +2 more
core  

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

open access: yesFEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim   +11 more
wiley   +1 more source

Between Humans and Machines: The social construction of the generative AI category

open access: yesOrganization Theory
ChatGPT’s debut in 2022 heralded the entry of generative AI into mainstream public attention. The radical technology could do what no machine had done before: mimic humans’ complex linguistic abilities. The ghost had entered the machine.
Stine Grodal   +3 more
doaj   +1 more source

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