Results 171 to 180 of about 431,994 (303)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Social Isolation and Loneliness Among U.S. Adults With Self-Reported Hospitalization. [PDF]

Am J Prev Med
Anguzu R, Sharda M, Amjad T, Pfankuch A, Iregbu S, Jha P, Kohlbeck S, Bhandari S.   +7 more
europepmc   +1 more source

Isolation rearing alters social behaviors and monoamine neurotransmission in the medial prefrontal cortex and nucleus accumbens of adult rats

, 2011
Isolation rearing induces profound behavioral and neurochemical abnormalities in rodents. However, there is some controversy regarding the effect of isolation rearing on social behaviors and monoamine neurotransmission in mesolimbic and mesocortical ...
Li, NX (reprint author), Yale Univ, Sch Med, Div Mol Psychiat, Dept Psychol, 34 Pk St, New Haven, CT 06508 USA., Wang, Weiwen, Shao, Feng, Han, Xiao, Li, Nanxin   +4 more
core  

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Redefining koinophilia: Solution to social isolation and polarization. [PDF]

Proc Natl Acad Sci U S A
Uzoigwe CE.
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Proceedings of the workshop 'Isolation of operators as a consequence of automation'

, 1987
'Isolation' beschreibt den physikalischen, sozialen, psychischen, professionellen und/oder kulturellen Zustand einer Person in ihrer Beziehung zu einem System. Mit dieser Thematik beschaeftigten sich 17 Fachbeitraege auf einer Arbeitstagung.
Deutsche Forschungsanstalt fuer Luft- und Raumfahrt e.V. (DLR), Koeln (Germany). Inst. fuer Flugmedizin, Commission of the European Communities (CEC), Brussels (Belgium), Boehm, H. (ed.)   +2 more
core  

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Gut microbiota restructured by social isolation: Evidence for sex-specific response patterns. [PDF]

Brain Behav Immun Health
Fallon K, Challa SR, Fornal CA, Boyineni J, Soares MB, Malchenko S, Veeravalli KK, Gyarmati P, Song Y.   +8 more
europepmc   +1 more source