Results 101 to 110 of about 758,685 (297)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

E-Commerce Platforms’ Cross-Platform Targeted Advertising Strategies: Cooperation with Social Media Platforms or Remaining Independent

Mathematics
E-commerce platforms are increasingly adopting cross-platform targeted advertising strategies, and the design of such strategies warrants attention. Focusing on cooperation between e-commerce and social media platforms, this study considers targeting ...
Fan Wu, Shue Mei, Weijun Zhong, Haiying Xu   +3 more
doaj   +1 more source

Oblique strategies for ambient journalism [PDF]

, 2010
Alfred Hermida recently posited ‘ambient journalism’ as a new framework for para- and professional journalists, who use social networks like Twitter for story sources, and as a news delivery platform.
Burns, Alex
core  

"With 1 follower I must be AWESOME :P". Exploring the role of irony markers in irony recognition

, 2018
Conversations in social media often contain the use of irony or sarcasm, when the users say the opposite of what they really mean. Irony markers are the meta-communicative clues that inform the reader that an utterance is ironic.
Ghosh, Debanjan, Muresan, Smaranda
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Idea Generation for New Service Development (NSD): Harnessing the Power of Social Media Platforms

Multidisciplinary Business Review, 2022
Customers frequently use social media (SM) platforms to share and seek information about services. Could the content of customer SM interactions be used for new service development (NSD)?
Neeraj Pandeya, Akshay Rupnawar
doaj  

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Understanding the role of social media in political corporate branding research in the context of Indian politics [PDF]

, 2015
This paper seeks to explore how political actors within the Bharatiya Janata Party (BJP) use social media in their communication campaigns and how these social media platforms are understood by citizens following the 2014 Indian General Election.
Armannsdottir, G, Ganesh, BE, Jain, V, Pich, C   +3 more
core  

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

US‐based and international environmental nongovernmental organizations use social media, but few have large audiences online

Conservation Science and Practice
Social media plays an outsized role in information dissemination, issue mobilization, and public influence. For environmental nongovernmental organizations (“eNGOs”), social media plays a critical role in fundraising and inspiring engaged audiences using
Hanna Kim, Paul R. Armsworth, Yuta J. Masuda, Charlotte H. Chang   +3 more
doaj   +1 more source