Results 221 to 230 of about 13,578,919 (257)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Resilience, Social Participation Including Mutual Support, and Subjective Quality of Life Among Community-Dwelling Older Adults in Japan: A Cross-Sectional Study. [PDF]

Healthcare (Basel)
Kazama J, Oohashi F, Kurabayashi S, Ohyama Y.   +3 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Digital and offline social participation configurations and depressive symptoms and life satisfaction among older adults in China. [PDF]

Front Public Health
Wei L, Tian Y, Chen H.
europepmc   +1 more source

The Persian Version of the Social Participation Questionnaire: Translation, Cross-Cultural Adaptation, and Psychometric Evaluation Using Categorical Principal Component Analysis (CATPCA): A Cross-Sectional Study. [PDF]

Health Sci Rep
Tori NA, Galeshi M, Sharif-Nia H, Ghoorchaiy S, Faghani S, Rezaei Z, Tirgar A, Moslemi D.   +7 more
europepmc   +1 more source

The Impact of Social Participation on Health Outcomes Among Middle-Aged and Older Adults in China: Evidence from the Chinese General Social Survey. [PDF]

Healthcare (Basel)
Zhou H, Xu H.
europepmc   +1 more source

Commentary on "Classification of Social Participation and Its Association With Loneliness in Older Adults: A Cross-Sectional Study of the National Center for Geriatrics and Gerontology-Study of Geriatric Syndromes". [PDF]

Geriatr Gerontol Int
Chen XY, Xu L.
europepmc   +1 more source

Association Between Social Participation, Physical Activity, and Intrinsic Capacity Decline: Empirical Evidence from the CHARLS. [PDF]

Healthcare (Basel)
Hu L, Tan J, Pu C.
europepmc   +1 more source